FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008126228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008126228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8097
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC616376.11461034951216No Hit
AGCACTTGAATCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC3604.44609114486847No Hit
AGCACTTGAAGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC2292.8282079782635545No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC951.1732740521180685No Hit
AGCACTTGAATCAACAGTGATCAGCAAAGACGAGACCAAGTCTCTGCTAC460.568111646288749No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC460.568111646288749No Hit
AGCACTTGAATCAACAAAGACGAGACCAAGTCTCTGCTACCGTACGGTGT390.48165987402741756No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC390.48165987402741756No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC370.4569593676670372No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC370.4569593676670372No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC340.41990860812646663No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC330.4075583549462764No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC280.3458070890453254No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC280.3458070890453254No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC270.33345683586513525No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC210.2593553167839941No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC210.2593553167839941No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC190.2346548104236137No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC190.2346548104236137No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC180.2223045572434235No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC170.20995430406323332No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC170.20995430406323332No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC160.19760405088304311No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC150.1852537977028529No Hit
AGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACCGTACGGTG150.1852537977028529No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCGAACTCACAC150.1852537977028529No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC140.1729035445226627No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC140.1729035445226627No Hit
AGCACTTGAATCAACAGCTTCAGAGACTGAGCCATTTTCAAACTCACACC120.14820303816228234No Hit
AGCACTTGATCAACAAAGACGAGACCAAGTCTCTGCTACCGTACGGTGTG110.13585278498209213No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC110.13585278498209213No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC100.12350253180190193No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC90.11115227862171174No Hit
AGCACTTGAATCAACGGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC90.11115227862171174No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)