FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008126286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008126286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8325
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC611573.45345345345345No Hit
AGCACTTGAATCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC3474.168168168168168No Hit
AGCACTTGAAGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC2933.5195195195195192No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC1041.2492492492492493No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC760.9129129129129129No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC520.6246246246246246No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC460.5525525525525525No Hit
AGCACTTGAATCAACAGTGATCAGCAAAGACGAGACCAAGTCTCTGCTAC450.5405405405405406No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC430.5165165165165164No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC380.45645645645645644No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC380.45645645645645644No Hit
AGCACTTGAATCAACAAAGACGAGACCAAGTCTCTGCTACCGTAGCATGC340.4084084084084084No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC320.3843843843843844No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC290.3483483483483484No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC250.3003003003003003No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC240.2882882882882883No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC210.25225225225225223No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC200.24024024024024024No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC200.24024024024024024No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC190.22822822822822822No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC190.22822822822822822No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC190.22822822822822822No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC180.21621621621621623No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC170.2042042042042042No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC160.1921921921921922No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC160.1921921921921922No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC150.18018018018018017No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC150.18018018018018017No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC140.16816816816816818No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTATCAAACTCACAC130.15615615615615616No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAACCATTTTCAAACTCACAC130.15615615615615616No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC130.15615615615615616No Hit
AGCACTTGAATCAACAGTCTTCAGAGACTGAGCCATTTTCAAACTCACAC120.14414414414414414No Hit
AGCACTTGATCAACAAAGACGAGACCAAGTCTCTGCTACCGTAGCATGCC120.14414414414414414No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC110.13213213213213215No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCAAACTCACACC110.13213213213213215No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCTCAC100.12012012012012012No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCGAACTCACAC100.12012012012012012No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGTGCCATTTTCAAACTCACAC90.10810810810810811No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCACTTTCAAACTCACAC90.10810810810810811No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)