FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008126294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008126294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8070
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC618076.57992565055763No Hit
AGCACTTGAATCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC2092.5898389095415117No Hit
AGCACTTGAAGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTAC1842.2800495662949194No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC931.1524163568773234No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC690.8550185873605948No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC510.6319702602230484No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC460.5700123915737298No Hit
AGCACTTGAATCAACAAAGACGAGACCAAGTCTCTGCTACCGTATCGAAC440.5452292441140025No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC390.483271375464684No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC340.4213135068153655No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC320.3965303593556382No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC310.38413878562577447No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC290.3593556381660471No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC290.3593556381660471No Hit
AGCACTTGAATCAACAGTGATCAGCAAAGACGAGACCAAGTCTCTGCTAC280.3469640644361834No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC240.29739776951672864No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC240.29739776951672864No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC200.24783147459727387No Hit
AGCACTTGATCAACAAAGACGAGACCAAGTCTCTGCTACCGTATCGAACT200.24783147459727387No Hit
AGCAACAATGATCAGCAAAGACGAGACCAAGTCTCTGCTACCGTATCGAA190.23543990086741015No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC180.22304832713754646No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC180.22304832713754646No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC170.21065675340768275No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC160.1982651796778191No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC160.1982651796778191No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC160.1982651796778191No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC160.1982651796778191No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC150.18587360594795538No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC150.18587360594795538No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC140.1734820322180917No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTATCAAACTCACAC140.1734820322180917No Hit
AGCACTTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACA130.161090458488228No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC130.161090458488228No Hit
AGCACTTGAATCAACGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC120.14869888475836432No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCGAACTCACAC120.14869888475836432No Hit
AGCACTTGAATCAACAGGCCTCAGAGACTGAGCCATTTTCAAACTCACAC120.14869888475836432No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCATAC110.13630731102850063No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCACTTTCAAACTCACAC110.13630731102850063No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAGCTCACAC110.13630731102850063No Hit
AGCACTTGAATCAACAGGCTTCAGAGATTGAGCCATTTTCAAACTCACAC100.12391573729863693No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC100.12391573729863693No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATATTCAAACTCACAC100.12391573729863693No Hit
AACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC90.11152416356877323No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAT90.11152416356877323No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)