FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008126339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008126339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5942
Total Bases861.4 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT379463.85055536856278No Hit
TGCTGATCATTGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA2814.7290474587680915No Hit
TGCTGATCATTGTTGCTTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA2434.089532144059239No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC651.093907775159879No Hit
TGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATCT410.6900033658700775No Hit
TGCTGATCACTGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA400.6731740154830024No Hit
CTCATCCTTGTCCACCGAAGATCCCTTTGTCACTGCAGCTTCTCTAGTGT350.589027263547627No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCAGGAGTATTTCCT250.4207337596768765No Hit
GCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC230.38707505890272637No Hit
TGTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC230.38707505890272637No Hit
TGCTGATATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC230.38707505890272637No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCAT220.3702457085156513No Hit
TGCTATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC210.35341635812857625No Hit
TGCTGTCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC190.31975765735442613No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATCTCCT160.26926960619320095No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAGGTCTGGAGTATTTCCT160.26926960619320095No Hit
TGCTGATCATTGTTGCTTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCC160.26926960619320095No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTACTTCCT150.2524402558061259No Hit
TGCTGATCATTGTTGCTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC150.2524402558061259No Hit
TGCTGATCATTTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC140.23561090541905083No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTCCCT140.23561090541905083No Hit
TGCTGATCATGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC130.21878155503197577No Hit
TGCTGATCATTGTTGCTTTGGGGGGGTGAGGAAAAGTCTGGAGTATTTCC130.21878155503197577No Hit
TGTTGATCAAGTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATCTC130.21878155503197577No Hit
TGCTGATCATTGTTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATC130.21878155503197577No Hit
TGCTGATCATTGGTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT120.2019522046449007No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAGAAGTCTGGAGTATTTCCT120.2019522046449007No Hit
TCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC120.2019522046449007No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAGAGTCTGGAGTATTTCCT110.18512285425782565No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA110.18512285425782565No Hit
TGCTGATCTTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.18512285425782565No Hit
TTGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCC110.18512285425782565No Hit
TGCTGATCATTGTTGCTTTGGGGGGAGAGGAAAAGTCTGGAGTATTTCCT110.18512285425782565No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTACT100.1682935038707506No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTTTTTCCT100.1682935038707506No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT100.1682935038707506No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTACCT90.15146415348367553No Hit
TGCTGATCATTGTTGTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.15146415348367553No Hit
TGTTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT90.15146415348367553No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCC90.15146415348367553No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCCGGAGTATTTCCT90.15146415348367553No Hit
TGCTGATCATAGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT90.15146415348367553No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGGGGAAAAGTCTGGAGTATTTCCT90.15146415348367553No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGAAGTATTTCCT80.13463480309660047No Hit
TGCTGACATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC80.13463480309660047No Hit
TGCTGATCAATGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT80.13463480309660047No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTGTTTCCT80.13463480309660047No Hit
TGCTGATCATTGATGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT70.11780545270952542No Hit
TGCTGATCATTGTTGCTTTGGTGGGTGAGGAAAAGTCTGGAGTATTTCCT70.11780545270952542No Hit
TGCTGATCATTGTTGCTTTGGGAGGTGAGGAAAAGTCTGGAGTATTTCCT70.11780545270952542No Hit
TGCTGATCATTGTTGCTTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT70.11780545270952542No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGCCTGGAGTATTTCCT60.10097610232245036No Hit
TGCTGATCATTGTTGCTTTGGGGGGCGAGGAAAAGTCTGGAGTATTTCCT60.10097610232245036No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGCATTTCCT60.10097610232245036No Hit
TGCTGATCATTGTTGCTATGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT60.10097610232245036No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTCTGTCCT60.10097610232245036No Hit
TGCTGATCATTGTTGATTCAAGTGCTTCGTGTGTAGAACCATGTCGTCAG60.10097610232245036No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)