FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008126545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008126545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8884
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC657373.9869428185502No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT1301.4633048176497072No Hit
TGCTGATCATTGTTGCTTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA480.5402971634398919No Hit
TGCTGATCATTGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCA450.5065285907248986No Hit
TGCTGATCATTGTTGCTTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTC370.4164790634849167No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT350.3939666816749212No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT350.3939666816749212No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT340.3827104907699235No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT330.3714542998649257No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC300.3376857271499325No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC300.3376857271499325No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT300.3376857271499325No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC290.3264295362449347No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT270.3039171544349392No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCAGGAGTATTTCCTC260.2926609635299415No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT260.2926609635299415No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC250.28140477262494373No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC250.28140477262494373No Hit
TGTTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC250.28140477262494373No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC230.2588923908149482No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC230.2588923908149482No Hit
TGCTGATCAATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC230.2588923908149482No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT220.24763619990995048No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC220.24763619990995048No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT210.23638000900495273No Hit
TGTTGATTCAAGTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATCT200.22512381809995496No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC200.22512381809995496No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTTGAGTATTTCCTC190.21386762719495722No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT190.21386762719495722No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC190.21386762719495722No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC190.21386762719495722No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTGTTTCCTC180.20261143628995948No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGGAAAGTCTGGAGTATTTCCTC180.20261143628995948No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT180.20261143628995948No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT170.19135524538496174No Hit
TGCTGATCATTGATGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC160.18009905447996397No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT150.16884286357496625No Hit
TGCTGATCATAGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC140.15758667266996848No Hit
TTGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT140.15758667266996848No Hit
TGTTGATCAAGTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATCTC140.15758667266996848No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC140.15758667266996848No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGCATTTCCTC140.15758667266996848No Hit
TGCTGATCATTGGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC130.14633048176497074No Hit
TGCTGATCATTGTTCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.14633048176497074No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGAAGTATTTCCTC110.12381809995497524No Hit
TGCAGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.12381809995497524No Hit
TGCTGATCATTGTTGCTTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT100.11256190904997748No Hit
TGCTGACATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT100.11256190904997748No Hit
TGCTGATCATCGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.10130571814497974No Hit
TGCTGATCATTGTTGCTTTCGTGTGTAGAACCATGTCGTCAGTGTAGATC90.10130571814497974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)