FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008126658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008126658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214
Total Bases175.1 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC61250.41186161449753No Hit
AGCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTAC17314.250411861614499No Hit
AGCACTTGAAGCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTAC1098.978583196046129No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC806.589785831960461No Hit
AGCACTTGAATCAACAGTGATCAGCATTACGCAGACCAAGTCTCTGCTAC393.2125205930807246No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC161.3179571663920924No Hit
AGCACTTGAATCAACAAGCTGCAGAGACTGAGCCATTTTCAAACTCACAC90.7413509060955519No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC60.4942339373970346No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC50.41186161449752884No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC50.41186161449752884No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC40.3294892915980231No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC40.3294892915980231No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC40.3294892915980231No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCGAACTCACAC40.3294892915980231No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC30.2471169686985173No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC30.2471169686985173No Hit
AGCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGACTCTGCTAC30.2471169686985173No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC30.2471169686985173No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC30.2471169686985173No Hit
AGCACTTGAAGCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC30.2471169686985173No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC30.2471169686985173No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAGACTCACAC30.2471169686985173No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAACCATTTTCAAACTCACAC30.2471169686985173No Hit
GCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTACC20.16474464579901155No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCAAACTCACACC20.16474464579901155No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC20.16474464579901155No Hit
AGCACTTGAATCAACAATGATCAGATTACGCAGACCAAGTCTCTGCTACC20.16474464579901155No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCACTTTCAAACTCACAC20.16474464579901155No Hit
AGCACTTGAATTAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC20.16474464579901155No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC20.16474464579901155No Hit
AGCACTTAAAGCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTAC20.16474464579901155No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCTCAC20.16474464579901155No Hit
AGCACTTGAATCAACAATGAATCAGCATTACGCAGACCAAGTCTCTGCTA20.16474464579901155No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)