FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008126663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008126663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7567
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT268935.535879476675035No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC255333.73860182370821No Hit
TGCTGATCATTGTTGATTCAAGTGCTCCTCTATGTAGAACCATGTCGTCA4395.801506541562046No Hit
TGCTGATCATTGTTGCTTCAAGTGCTCCTCTATGTAGAACCATGTCGTCA1491.9690762521474825No Hit
TGCTGATCACTGTTGATTCAAGTGCTCCTCTATGTAGAACCATGTCGTCA740.9779304876437162No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT220.2907360909211048No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAGGTCTGGAGTATTTCCT180.2378749834809039No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC150.19822915290075327No Hit
TGCTATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC150.19822915290075327No Hit
TGCTGATCATTGTTGCTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC150.19822915290075327No Hit
TGCTGATCATGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC140.18501387604070307No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGGGGAAAAGTCTGGAGTATTTCCT140.18501387604070307No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.17179859918065285No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCAT130.17179859918065285No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCCGGAGTATTTCCT120.15858332232060263No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCAGGAGTATTTCCT120.15858332232060263No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT110.1453680454605524No Hit
TGTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.1453680454605524No Hit
TGCTGATATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.1453680454605524No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT110.1453680454605524No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC100.13215276860050218No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC100.13215276860050218No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC100.13215276860050218No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC100.13215276860050218No Hit
TGCTGATCATTGTTGCTTTGGGGGGGTGAGGAAAAGTCTGGAGTATTTCC90.11893749174045196No Hit
TGCTGTCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.11893749174045196No Hit
TGCTGATCATTGTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC90.11893749174045196No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT90.11893749174045196No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC80.10572221488040175No Hit
TGCTGATCAATGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT80.10572221488040175No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTAGTGCCTC80.10572221488040175No Hit
GCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC80.10572221488040175No Hit
TGCTGATTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT80.10572221488040175No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT80.10572221488040175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)