FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008126706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008126706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5805
Total Bases836.6 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC393367.75193798449612No Hit
AGCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTAC3716.391042204995694No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC2904.995693367786391No Hit
AGCACTTGAAGCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTAC1993.4280792420327306No Hit
AGCACTTGAATCAACAGTGATCAGCATTACGCAGACCAAGTCTCTGCTAC641.102497846683893No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC480.8268733850129198No Hit
AGCACTTGAATCAACAAGCTGCAGAGACTGAGCCATTTTCAAACTCACAC370.6373815676141257No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC320.5512489233419465No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC270.46511627906976744No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC250.4306632213608958No Hit
AGCACTTGAATCACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC230.3962101636520241No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC220.3789836347975883No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC190.32730404823428083No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC190.32730404823428083No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC180.31007751937984496No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC160.27562446167097326No Hit
AGCACTTGAATCAACAGGCTTCAGTGACTGAGCCATTTTCAAACTCACAC160.27562446167097326No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC160.27562446167097326No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC140.24117140396210163No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC140.24117140396210163No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC140.24117140396210163No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC140.24117140396210163No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC140.24117140396210163No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC120.20671834625322996No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC110.18949181739879414No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC110.18949181739879414No Hit
AGCACTTGAATCAACAGCTTCAGAGACTGAGCCATTTTCAAACTCACACC100.17226528854435832No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTATCAAACTCACAC100.17226528854435832No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC100.17226528854435832No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC90.15503875968992248No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC90.15503875968992248No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCGTTTTCAAACTCACAC80.13781223083548663No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCACTTTCAAACTCACAC80.13781223083548663No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCAAACTCACACC80.13781223083548663No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCAATTTCAAACTCACAC80.13781223083548663No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC70.12058570198105081No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGTGCCATTTTCAAACTCACAC70.12058570198105081No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCGAACTCACAC70.12058570198105081No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC60.10335917312661498No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTCTCAAACTCACAC60.10335917312661498No Hit
AGCACTTGAATTAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC60.10335917312661498No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC60.10335917312661498No Hit
AGCACTTGAATCAACAGGCTTTAGAGACTGAGCCATTTTCAAACTCACAC60.10335917312661498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)