FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008126714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008126714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493
Total Bases71.2 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTAC16232.86004056795132No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC14930.223123732251523No Hit
AGCACTTGAAGCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTAC8116.43002028397566No Hit
AGCACTTGAATCAACAGTGATCAGCATTACGCAGACCAAGTCTCTGCTAC204.056795131845842No Hit
AGCACTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACAC71.4198782961460445No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC71.4198782961460445No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC20.4056795131845842No Hit
AGCACTTGAAGCAACAATGATAGCATTACGCAGACCAAGTCTCTGCTACC20.4056795131845842No Hit
AGCACTTGAATCAACAACGATCAGCATTACGCAGACCAAGTCTCTGCTAC20.4056795131845842No Hit
AGCACTTGAATCAACAATGATCAGCATTACTCAGACCAAGTCTCTGCTAC20.4056795131845842No Hit
AGCACTTGAATCAACAGGCTTCAGAGATTGAGCCATTTTCAAACTCACAC20.4056795131845842No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC20.4056795131845842No Hit
TGCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTAC20.4056795131845842No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC20.4056795131845842No Hit
ACACTTGAAGCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTACC20.4056795131845842No Hit
AGCACTTGAAGCAACAATATCAGCATTACGCAGACCAAGTCTCTGCTACC10.2028397565922921No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCCCAC10.2028397565922921No Hit
AGCACTTGAATCCAGGCTTCAGAGACTGCGCCATTTTCAAACTCACACCT10.2028397565922921No Hit
AGCACTTGAATCAACAGTCATCAGCATTACGCAGACCAAGTCACTGCTAC10.2028397565922921No Hit
AGCACTTGAAGCAACAATGATCAGAATTACGCAGACCAAGTCTCTGATAC10.2028397565922921No Hit
AGCACTTGAATCAACAGGCTTCAGACACTGAGCCATTTTCAAACTCACAC10.2028397565922921No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATCTTCAAACTCACAC10.2028397565922921No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTTACAC10.2028397565922921No Hit
AGCACTTGAAGCAACAATGTCAGCATTACGCAGACCAAGTCTCTGCTACC10.2028397565922921No Hit
AGCTTGAATCAACAGGCTGCAGAGACTGAGCCATTTTCAAACTCACACCT10.2028397565922921No Hit
AGCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGACACTGCAAC10.2028397565922921No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC10.2028397565922921No Hit
AGCATTTGAATCAACAATGATCAACATTACGCAGACCAAGTCTCTGCTAC10.2028397565922921No Hit
AGCACTTGAAGCAACAATGATCAGCATTACGCAGACAAAGTCTCTGCTAC10.2028397565922921No Hit
AGCACTTGAATCAACAATGAACAGCATTACGCAGACAAAGTCACTGCTAC10.2028397565922921No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAT10.2028397565922921No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC10.2028397565922921No Hit
AGCATTGAATCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTACC10.2028397565922921No Hit
AGCACTTGAATCAACAGGCACACTGACGACATGGTTCTACATAGAGGAGC10.2028397565922921No Hit
AGCACTTGAAGCAACAATGATCAGCATTACGCAGACCAAGACTCTGATAC10.2028397565922921No Hit
AGCACTTGAATCAACAGGCTACAGAGACTGAGCCATTTTCAAACTCACAC10.2028397565922921No Hit
AGCACTTGAAGCAACAATGATCACATTACGCAGACCAAGTCTCTGCTACC10.2028397565922921No Hit
ACACTTGAATCAACAGTGATCAGCATTACGCAGACCAAGTCTCTGCTACC10.2028397565922921No Hit
AGCACTTGAATCAACAATGATCAGATTACGCAGACCAAGTCTCTGCTACC10.2028397565922921No Hit
AGCACTGAAGCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTACC10.2028397565922921No Hit
AGCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGTCACTGCTAC10.2028397565922921No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC10.2028397565922921No Hit
AGCACTTGATCAACAGGCCTCAGAGACTGAGCCATTTTCAAACTCACACC10.2028397565922921No Hit
AGCACTTGAATCAACAGGCTCCAGAGACTGAGCCATTTTCAAACTCACAC10.2028397565922921No Hit
AGCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGTCTCTCCTAC10.2028397565922921No Hit
AGCACTTGAATCAACAAGCTACAGAGACTCAGCCATTATCAAACTCACAC10.2028397565922921No Hit
AGCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGACTCTGCTAC10.2028397565922921No Hit
AGCACTTGAATCAACAGGCTGCAGAGACCGAGCCATTTTCAAACTCACAC10.2028397565922921No Hit
AGACTTGAATCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTACC10.2028397565922921No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTACAAACTCACAC10.2028397565922921No Hit
AGCACTTGAATCAACAAGCTAAGCAGTGGTATCAACGCAGAGTACATGGA10.2028397565922921Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGACTTGAATCAACAGTGATCAGCATTACGCAGACCAAGTCTCTGCTACC10.2028397565922921No Hit
AGCACTTGAATCAACAGGCTGCACAAACACAACCATCCCAGAGTTCACAT10.2028397565922921No Hit
AGCACTTGAATCAACAAAGATCAGCATTACGCAGACCAAGTCTCTGCTAC10.2028397565922921No Hit
AGCACTTGAATCAACAATGATCATCATTACGCAGACCAAGTCTCTGCTAC10.2028397565922921No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAATCTCACAC10.2028397565922921No Hit
AGCATTGAAGCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTACC10.2028397565922921No Hit
AGCACTTGAATCAACAGGCTTCAGAGACAGAGCCATTTTCAAACTCACAC10.2028397565922921No Hit
AGCCTTGAAGCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTACC10.2028397565922921No Hit
AGCACTTGAGCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTACC10.2028397565922921No Hit
AGTACTTGAAGCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTAC10.2028397565922921No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCACC10.2028397565922921No Hit
AGCACTTGAATCAACAATGATCAGCATTACGCACACAAAGACACTGCTAC10.2028397565922921No Hit
AGCACTTGAATCAACAATGATAGCATTACGCAGACCAAGTCTCTGCTACC10.2028397565922921No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)