FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008126717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008126717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6356
Total Bases921.4 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC424166.72435494021397No Hit
TGCTGATCATTGTTGATTCAAGTGCTCCTCTATGTAGAACCATGTCGTCA5568.747640025173064No Hit
TGCTGATCATTGTTGCTTCAAGTGCTCCTCTATGTAGAACCATGTCGTCA1983.1151667715544367No Hit
TGCTGATCACTGTTGATTCAAGTGCTCCTCTATGTAGAACCATGTCGTCA590.9282567652611706No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT340.5349276274386406No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT300.4719949653870359No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT270.4247954688483323No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT240.3775959723096287No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT210.3303964757709251No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT200.3146633102580239No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT180.28319697923222154No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC180.28319697923222154No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT150.23599748269351795No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC150.23599748269351795No Hit
TGCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT140.22026431718061676No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC140.22026431718061676No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT120.18879798615481436No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT120.18879798615481436No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC110.17306482064191317No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC110.17306482064191317No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC110.17306482064191317No Hit
TGCTGATCATTGTTGTTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.15733165512901195No Hit
TGCTGACATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT100.15733165512901195No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTAGTGCCTC100.15733165512901195No Hit
TGCTGATCATTGATGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC100.15733165512901195No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC90.14159848961611077No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC90.14159848961611077No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGGAAAGTCTGGAGTATTTCCTC90.14159848961611077No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTGTTTCCTC80.12586532410320958No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC80.12586532410320958No Hit
TGCTGATCCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC80.12586532410320958No Hit
TGCTGATCATTGGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC80.12586532410320958No Hit
TGCTGATCAATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC80.12586532410320958No Hit
TGCTGATCATAGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC70.11013215859030838No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT70.11013215859030838No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTGCTC70.11013215859030838No Hit
TGCTGATCATTGTTGATTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC70.11013215859030838No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)