FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008126944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008126944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270
Total Bases39.1 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTAC14654.074074074074076No Hit
AGCACTTGAAGCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTAC5620.74074074074074No Hit
AGCACTTGAATCAACAGTGATCAGCATTACGCAGACCAAGTCTCTGCTAC238.518518518518519No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC93.3333333333333335No Hit
AGCACTTGAAGCAACAATGATCAGCATTACGCAGACCAAGTCACTGCTAC20.7407407407407408No Hit
AGCACTTGAATCAACAATTATCAGCATTACGCAGACCAAGTCTCTGCTAC20.7407407407407408No Hit
AGCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGTCACTGCTAC20.7407407407407408No Hit
AGCACTTGAATCAAAATGATCAGCATTACGCAGACCAAGTCTCTGCTACC20.7407407407407408No Hit
AGCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGACTCTGCTAC20.7407407407407408No Hit
AGCACTTGAATCAACAGGCTTTAGAGACTGAGCCATTTTCAAACTCACAC10.3703703703703704No Hit
AGCACTTGAATCAACAGTGATCAACATTACGCAGACCAAGTCTCTGCTAC10.3703703703703704No Hit
AGCATTGAATCAACAGTGATCAGCATTACGCAGACCAAGTCTCTGCTACC10.3703703703703704No Hit
AGCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGTCTCAGCTAC10.3703703703703704No Hit
AGCACTTGAAGCAACAATGATCACATTACGCAGACCAAGTCTCTGCTACC10.3703703703703704No Hit
AGCACTTGAATCAACAGGATCAGCATTACGCAGACCAAGTCTCTGCTACC10.3703703703703704No Hit
AGCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTTC10.3703703703703704No Hit
AGCACTTGAATCAGCATTACGCAGACCAAGTCTCTGCTACCGTAGTAGTA10.3703703703703704No Hit
AGCACTTGAATCAACAAAGATCAGCATTACTCAGACCAAGTCTCTGCTAC10.3703703703703704No Hit
AGCACTTGAAGCACAATGATCAGCATTACGCAGACCAAGTCTCTGCTACC10.3703703703703704No Hit
GCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTACC10.3703703703703704No Hit
AGCACTTGAATCAACATGATCAGCATTACGCAGACCAAGTCTCTGCTACC10.3703703703703704No Hit
AGCACTTGAAGCAACAATGACAGCATTACGCAGACCAAGTCTCTGCTACC10.3703703703703704No Hit
AGCACTTGAATCAACAGTGATCAGCATTACGCAGACCAAGTCACTGCTAC10.3703703703703704No Hit
AGCACTTGAATCAACAATGATCAGCATTACGCAGACCAAGACTCAGCTAC10.3703703703703704No Hit
AGCACTTGAATCAACAATCATCAGCATTACGCAGACCAAGTCTCTGCTAC10.3703703703703704No Hit
AGCACTTGAATCAACAATGACAGCATTACGCAGACCAAGTCTCTGCTACC10.3703703703703704No Hit
AGCACTTGAAGCAACAATGTCAGCATTACGCAGACCAAGTCTCTGCTACC10.3703703703703704No Hit
AGCACTTGAATCACAATGATCAGCATTACGCAGACCAAGTCTCTGCTACC10.3703703703703704No Hit
AGCACTTGAATCAACAATATCAGCATTACGCAGACCAACTCACAGCAACC10.3703703703703704No Hit
AGCACTTGAATCAACAATGATCACATTACGCATCACAAGTGTAATCTTAC10.3703703703703704No Hit
AGCACTTGAATCAACACTGATCAGCATTACGCAGACGAAGTCTCTGCTAC10.3703703703703704No Hit
AGCACTTGAAGCAACAATGATCAGTATTACTCAGACCAAGTCTCTGCTAC10.3703703703703704No Hit
AGCACTTGAAGCAACAATGATAGCATTACGCAGACCAAGTCTCTGCTACC10.3703703703703704No Hit
ACACTTGAAGCAACAATGATCAGCATTACGCAGACCAAGTCTCTGCTACC10.3703703703703704No Hit
AGCACTTGAATCAACAATGATCGGCATTACGCAGACCAAGTCTCTGCTAC10.3703703703703704No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)