FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008127077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008127077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9066
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length144-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC796287.82263401720715No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT720.7941760423560555No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT500.5515111405250386No Hit
TGCTGATCATTGTTGATTCAAGTGCTTCACAGTGTAGAACCATGTCGTCA380.4191484667990294No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC290.31987646150452237No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT270.29781601588352086No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT260.2867857930730201No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT240.26472534745201853No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC220.24266490183101697No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT220.24266490183101697No Hit
TGCTGATCATTGTTGCTTCAAGTGCTTCACAGTGTAGAACCATGTCGTCA210.2316346790205162No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC210.2316346790205162No Hit
TGCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT200.22060445621001545No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC190.2095742333995147No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC190.2095742333995147No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT190.2095742333995147No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT170.18751378777851313No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.17648356496801235No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC150.1654533421575116No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGCATTTCCTC140.1544231193470108No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC140.1544231193470108No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAATATTTCCTC140.1544231193470108No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC140.1544231193470108No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT140.1544231193470108No Hit
TGCTGATCTTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT140.1544231193470108No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC130.14339289653651005No Hit
TGCTGATCATTGTTGTTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC120.13236267372600927No Hit
TGCTGACATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT120.13236267372600927No Hit
TGCTGATCATTGTTGCTTTGGGGGCGAGGAAAAGTCTGGAGTATTTCCTC120.13236267372600927No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT120.13236267372600927No Hit
TGCTGATCATTGTTGTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT120.13236267372600927No Hit
TACTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC110.12133245091550848No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT110.12133245091550848No Hit
TGCTGATCATTGTTGCTTTGGGAGTGAGGAAAAGTCTGGAGTATTTCCTC110.12133245091550848No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC100.11030222810500773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)