FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008127172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008127172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10485
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC889684.84501669051025No Hit
AGCACTTGAATCAACAATGATCAGCACCATGTAGACCAAGTCTCTGCTAC3823.6432999523128275No Hit
AGCACTTGAAGCAACAATGATCAGCACCATGTAGACCAAGTCTCTGCTAC950.9060562708631379No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC490.4673342870767763No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC440.41964711492608486No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC440.41964711492608486No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC360.34334763948497854No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC360.34334763948497854No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC350.33381020505484027No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC300.28612303290414876No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC280.2670481640438722No Hit
AGCACTTGAATCAACAGTGATCAGCACCATGTAGACCAAGTCTCTGCTAC280.2670481640438722No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC280.2670481640438722No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCAAACTCACACC270.2575107296137339No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC270.2575107296137339No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC240.22889842632331905No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC230.21936099189318073No Hit
AGCACTTGAATCAACGGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC220.20982355746304243No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAACCATTTTCAAACTCACAC190.18121125417262757No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC180.17167381974248927No Hit
AGCACTTGAATCAACAGGCTTCAGAGACCGAGCCATTTTCAAACTCACAC180.17167381974248927No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC160.15259895088221268No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTATCAAACTCACAC150.14306151645207438No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC140.1335240820219361No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCCAAACTCACAC130.12398664759179781No Hit
AGCCTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC130.12398664759179781No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC130.12398664759179781No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC120.11444921316165953No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCGAACTCACAC120.11444921316165953No Hit
AGCACTTGAATCAACAGACTTCAGAGACTGAGCCATTTTCAAACTCACAC120.11444921316165953No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC120.11444921316165953No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCATAC110.10491177873152122No Hit
AGCACTTGAATCAACAGTCTTCAGAGACTGAGCCATTTTCAAACTCACAC110.10491177873152122No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)