FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008127176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008127176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9796
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC845286.28011433238056No Hit
AGCACTTGAATCAACAATGATCAGCACCATGTAGACCAAGTCTCTGCTAC3083.144140465496121No Hit
AGCACTTGAAGCAACAATGATCAGCACCATGTAGACCAAGTCTCTGCTAC640.6533278889342589No Hit
AGCACTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC530.5410371580236831No Hit
AGCACTTGATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC510.5206206614944875No Hit
GCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC400.4083299305839118No Hit
AGCATTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC350.35728868926092283No Hit
AGCACTTAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC300.30624744793793385No Hit
AGCACTTGAATAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC260.2654144548795427No Hit
AGACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC240.2449979583503471No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGGGCCATTTTCAAACTCACAC220.2245814618211515No Hit
AGCACTTGAATCAACAGGTTTCAGAGACTGAGCCATTTTCAAACTCACAC170.17354022049816253No Hit
AGCACTTGAATCAACAGGCTTCGGAGACTGAGCCATTTTCAAACTCACAC170.17354022049816253No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTCAAACTCACACC160.16333197223356472No Hit
AGCACTTGAATCAACAGTGATCAGCACCATGTAGACCAAGTCTCTGCTAC150.15312372396896692No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACCCACAC150.15312372396896692No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGTCATTTTCAAACTCACAC150.15312372396896692No Hit
AGCACTTGAACAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC150.15312372396896692No Hit
AGCACTTGAATCAACAAGCTTCAGAGACTGAGCCATTTTCAAACTCACAC140.14291547570436913No Hit
ACACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC140.14291547570436913No Hit
AGCACTTGAATCAACGGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC140.14291547570436913No Hit
AGCACTTGAATCAACAGTCTTCAGAGACTGAGCCATTTTCAAACTCACAC130.13270722743977134No Hit
AGCCTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACACC130.13270722743977134No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAACCATTTTCAAACTCACAC130.13270722743977134No Hit
AGCACTTGAATCAACAGGCTTCAGGGACTGAGCCATTTTCAAACTCACAC110.11229073091057575No Hit
AGCACTAGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC110.11229073091057575No Hit
AGCACTTGAATCAACAGGCCTCAGAGACTGAGCCATTTTCAAACTCACAC110.11229073091057575No Hit
AGCACTTGAATCAACAGGCTTCAGAGGCTGAGCCATTTTCAAACTCACAC110.11229073091057575No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCGCAC100.10208248264597795No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTATCAAACTCACAC100.10208248264597795No Hit
AGCACTTGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACGC100.10208248264597795No Hit
AGCACTCGAATCAACAGGCTTCAGAGACTGAGCCATTTTCAAACTCACAC100.10208248264597795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)