FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008127231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008127231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6523
Total Bases945.8 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTC558685.63544381419592No Hit
TGCTGATCATTGTTGATTCAAGTGCTTCACAGTGTAGAACCATGTCGTCA1592.437528744442741No Hit
TGCTGATCATTGTTGCTTTGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT520.7971792120190095No Hit
TGCTGATCATTGTTGCTTCAAGTGCTTCACAGTGTAGAACCATGTCGTCA370.5672236700904493No Hit
TGCTGATCATTGTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT240.3679288670856968No Hit
TGCGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT220.33726812816188867No Hit
TGCTGATATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT200.3066073892380806No Hit
TGTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT190.2912770197761766No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATCTCCTC190.2912770197761766No Hit
TGCTATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT190.2912770197761766No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTACTTCCTC180.27594665031427257No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTCCCTC170.26061628085236854No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGGTCTGGAGTATTTCCTC160.24528591139046452No Hit
TCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT160.24528591139046452No Hit
TGCTGATCATTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT150.2299555419285605No Hit
TGCTGATCATTGTTGCTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT140.21462517246665647No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAGAGTCTGGAGTATTTCCTC130.19929480300475239No Hit
GCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT130.19929480300475239No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAGAAGTCTGGAGTATTTCCTC120.1839644335428484No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCCGGAGTATTTCCTC120.1839644335428484No Hit
TGCTGTCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT120.1839644335428484No Hit
TGCTGATCATTGTTGCTTTGGGGGTGGGGAAAAGTCTGGAGTATTTCCTC110.16863406408094433No Hit
TGCTGATCATTGTTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCT110.16863406408094433No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGGGTATTTCCTC110.16863406408094433No Hit
TGCTGACATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT100.1533036946190403No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGCCTGGAGTATTTCCTC100.1533036946190403No Hit
TGCTGATCATGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT90.13797332515713628No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCCC80.12264295569523226No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAGTCTGGAGTATTTCCTCT80.12264295569523226No Hit
TGCTGATCATTGTTCTTTGGGGGTGAGGAAAAGTCTGGAGTATTTCCTCT80.12264295569523226No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAGTGTTTCCTC80.12264295569523226No Hit
TGCTGATCATTGTTGCTTTGGGGGTGAGGAAAAGTCTGGAATATTTCCTC70.10731258623332823No Hit
TGCTGATCATTGTTGCTTTGGGGGGTGAGGAAAAGTCTGGAGTATTTCCT70.10731258623332823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)