FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008127338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008127338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263
Total Bases38.1 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG21280.6083650190114No Hit
TGGGTCATGTGGTTCGGAGGCTTACACTTGGGTCTTAGACCAAGTCTCTG72.6615969581749046No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA31.1406844106463878No Hit
CTTCCAGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA20.7604562737642585No Hit
CTTCCAGGCCTCCGAGGCTTACACTTGGGTCTTAGACCAAGTCTCTGCTA20.7604562737642585No Hit
CTTCCAGGCCTCCTAGTGCTTACGACAGCGATGAGAGTGACAGGTCTTAG10.38022813688212925No Hit
CTTCCGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA10.38022813688212925No Hit
CATCCAGGCCTCCTAGTGGTTGTCTTTTGCAATTAAAAAAATGAAGTGAC10.38022813688212925No Hit
CTTCCAGGCCTCCTAGCGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG10.38022813688212925No Hit
TGGGTCTGTGGTTCGGAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT10.38022813688212925Clontech Universal Primer Mix Long (96% over 25bp)
CTTCCAGGCCTCCAAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG10.38022813688212925No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGTAGGG10.38022813688212925No Hit
TGGGTCATGTGGTTCGGAGAACCAAGCGATGAGAGTGACAGGTCTTAGAC10.38022813688212925No Hit
CATCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG10.38022813688212925No Hit
TGGGTCATGTGGTTCGGAGGCTTACACTGGGTCTTAGACCAAGTCTCTGC10.38022813688212925No Hit
TGGGTCATGTGGTTCGGAGAGTACATGGGAAGCAGTGGTATCAACGCAGA10.38022813688212925Clontech Universal Primer Mix Long (100% over 21bp)
CTTCCAGGCCTCCTAGTGTGCTGGGATTACAGGCGTGAGCCACCACGCCC10.38022813688212925No Hit
CTTCCAGGCCTCCTAGTGCTCAAGGCTCCTCCTCCGAGCTCCCAGCAGGG10.38022813688212925No Hit
CTTCCAGGCCTCCTTGACCTTGGGCTTCTCTGCGTTGATACCACTGCTTC10.38022813688212925Clontech SMART CDS Primer II A (95% over 24bp)
CTTCCAGCCCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGAT10.38022813688212925No Hit
TGGGTCATGTGGTTCGGACAGGGAGGCAGGGAGGCAGGGAGAGACAGAGA10.38022813688212925No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCGCAGCAGGG10.38022813688212925No Hit
TGGGTCATGTGGTTCGGAGAGGACAGATAGTAGAGGCCAATCACCCGGTA10.38022813688212925No Hit
CTTCCAGGCCTCCTAGGCTTACACTTGGGTCTTAGACCAAGTCTCTGCTA10.38022813688212925No Hit
TGGGTCATGTGGTTCGAGGCTTACACTTGGGTCTTAGACCAAGTCTCTGC10.38022813688212925No Hit
CTTTAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA10.38022813688212925No Hit
CTTCCAGGCCTCCTAGTGCTGACTTGAAGGCTGCTGCAGCTTCTCCTACT10.38022813688212925No Hit
CTTCCAGGCCTCCTAGTGCTTCTCAAAAACAAACAAGAACTTTCTAAGCA10.38022813688212925No Hit
CTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA10.38022813688212925No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCGGCAGGG10.38022813688212925No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTGCGAGCTCCCAGCAGGG10.38022813688212925No Hit
TGGGTCATGTGGTTCGGAGAAGCTTCACAGATAATTTACTACTAAAGAAC10.38022813688212925No Hit
CATCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCACCCAGCAGGG10.38022813688212925No Hit
CTTCCAGGCCTCCTAGTGGTTGTCTTTTGCAATTAAAAAAATGAAGTGAC10.38022813688212925No Hit
TGGGTCATGTGGTTCCGAGGCTTACACTTGGGTCTTAGACCAAGTCTCTG10.38022813688212925No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAACTCCCAGCAGGG10.38022813688212925No Hit
CTTCCAGGCCTCCTAGTGCGACAGCGATGAGAGTGACAGGTCTTAGACCA10.38022813688212925No Hit
TGGGTCATGTGGTTCGGAGAACCATGCGACAGCGATGAGAGTGACAGGTC10.38022813688212925No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCACCGAGCTCCCAGCAGGG10.38022813688212925No Hit
CTTCCAGGCCTCCTAGTGCCCCAAGGGTCCTTCCCCACTTTATGATACAT10.38022813688212925No Hit
CTTCCAGGCCTCCTAGTGCTCCAGGCTCCTCCTCCGAGCTCCCAGCAGGG10.38022813688212925No Hit
CTTCCAGGCCTCCTAGTTTCCTCCACCAAATCGCACTGGCTCCTGGACTC10.38022813688212925No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)