FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008127339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008127339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263
Total Bases38.1 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG17566.53992395437263No Hit
CAAGTGTAAGCCTCCGAACCACATGACCCACGAGTTTGTAGAACCATGTC51.9011406844106464No Hit
CAAGTGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGT41.520912547528517No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGTG31.1406844106463878No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTCTG31.1406844106463878No Hit
TGTCACTCTCATCGCTGTCGTCTGAGTAGTGAGGGTGGCATTCTTGTCCC20.7604562737642585No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT20.7604562737642585No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT20.7604562737642585No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT20.7604562737642585No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGAG10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCGAGGTGTGTG10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCGTGTCCCCCCAGGTGTGTG10.38022813688212925No Hit
CAAGTGTAAGCCTCGGAGGCCTGGAAGCGAGTTTGTAGAACCATGTCGTC10.38022813688212925No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGTT10.38022813688212925No Hit
CAAGTGTAAGCCTCCGAACCACATGCCCCACGAGTTTGTAGAACCATGTC10.38022813688212925No Hit
CAAGAGTAAGCCTAGGAACAACAGGGGGGCCCCGGGGGGCGGCGGCCCGC10.38022813688212925No Hit
CAAGTGTAAGCCTCGCAAAACAGGCCGGGCGTTGACCCCCCGGGGGTGTG10.38022813688212925No Hit
TGTCACTCTCATCGCTGTCGAAGCAGTGGTATCAACGCAGAGTACATGGA10.38022813688212925Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTCACTCTCATCGCTGTCGCACTAGGAGGCCTGGAAGCGAGTTTGTAGA10.38022813688212925No Hit
CAAGTCTAAGGGTGGCGTGGGGGGTTAGTCACCGGCTTTATGCGGGCCGA10.38022813688212925No Hit
CAAGTCTAATCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.38022813688212925No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGAGT10.38022813688212925No Hit
CAAGAGTATGCCTCCGAACCACATGACCCACGACTTTCTAGAACCATGTC10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCATAGTGGACATGGACGAGGGGGCAGCTTGTTTC10.38022813688212925No Hit
CAAGTTAAAGCCTCGGAGGCCTGGAAGCGAGTTTGTAGAACCATGTCGTC10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCCTTTTCTTGACACTCCTGTGTATTTTCTGTCTT10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCCTCCCCAAGTGTTGGGATTACAGGAGTTAGCCA10.38022813688212925No Hit
CAAGTGTAACCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT10.38022813688212925No Hit
CAAGTGTAAGACACGGCAAACAAGGACTGGCTTGTCCCCCCAGGTGTGTG10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGAG10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCTTTGTTGGCGTCGCTTGTGGGGCCAGGTGTGTG10.38022813688212925No Hit
TGTCACTCTCATCGCTGTCGCATGGTTCTCCGAACCACATGACCCACGAG10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT10.38022813688212925No Hit
CGAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.38022813688212925No Hit
CAGTGTAAGCCTCCGAACCACATGACCCACGAGTTTGTAGAACCATGTCG10.38022813688212925No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCCTGAGCCA10.38022813688212925No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT10.38022813688212925No Hit
CAACTGTTTGGGTGCGCATTGCGCGCGGGCGCCCGGGCGGGCCGCCGGCC10.38022813688212925No Hit
CAAGTGAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCA10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCAGACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGGGTGTG10.38022813688212925No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT10.38022813688212925No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCACGGTGTGTGT10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCAACAAGAGCAAAACTCCATCTCCAAAAAAAAAA10.38022813688212925No Hit
CAAGAGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCTAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.38022813688212925No Hit
CAAGTGTAAGCCTAGGAGGCCTGGAAGCGAGTTTGTAGAACCATGTCGTC10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCGCCCCAGGTGTGTG10.38022813688212925No Hit
CTAGTGTAAGCCTCGGCAAACAAGGCCTGGCTGGTCCCCCAGGTTGGGGA10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCCTCCCAAAGTGCTGGGATTACAGACAGGCGTGA10.38022813688212925No Hit
CAAGTGAAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGAGTG10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCCGGCTTGTCCCCCCAGGTGTGTG10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGACTTGTCCCCCCAGGTGTGTG10.38022813688212925No Hit
CAAGTGTAAGCCTCGACAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.38022813688212925No Hit
CAAGTGTAAGCCTCGAACCACATGACCCACGAGTTTGTAGAACCATGTCG10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGGTTGTCCCCCCAGGTGTGTG10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCCTCCCAAAGTGCTGGGATTACATACAGGAGTGA10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG10.38022813688212925No Hit
CAAGTGTCAGCCTGGGCCTCCCAAAGTGCTGGGATTACAGGCGAGAGCCA10.38022813688212925No Hit
CAAGTGTTAAGCCTCGGCAAACAAGGCCTAGCTTGTCCCCCCAGGTGTGT10.38022813688212925No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCCGGCTTGTCCCCCCAGGTGTGTGT10.38022813688212925No Hit
CAAGTGTAAGCATCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.38022813688212925No Hit
CAAGTGCAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.38022813688212925No Hit
CAAGTGTAAGCCACGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAATGTG10.38022813688212925No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCACCCCAGGTGTGTG10.38022813688212925No Hit
CAAGTGAAAGCCACCACAAACAAGGCCTGGCTAGTCCCCCCAGGACTGAG10.38022813688212925No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCTGGGGTGTGT10.38022813688212925No Hit
CAAGTGTAAGCCTCGGAAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.38022813688212925No Hit
TGTCACTCTCATCGCTGTCGTAAGCACTAGGAGGCCTGGAAGCGAGTTTG10.38022813688212925No Hit
CAGTGTTTGATGAAGCTAGGACTGAGGAGCAAGCGAGCAAGCAGCAGTTC10.38022813688212925No Hit
TGTCACGCGCAACGCTTGGGGCTCCGACCCACAAGACCCACGAGGAGGGA10.38022813688212925No Hit

[WARN]Adapter Content

Adapter graph