FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008127519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008127519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10974
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG531248.4053216694004No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG291726.58100965919446No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT780.7107709130672498No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT630.5740841990158557No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT620.5649717514124294No Hit
TGTCCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG550.5011846181884454No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT400.3644979041370512No Hit
CAAGTGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGT390.3553854565336249No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT350.3189356661199198No Hit
TGTCACTCTCATCCCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG320.29159832330964097No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGCCCCCCCAGGTGTGTG280.25514853289593586No Hit
TGTCACTCTCATCGCTGTCGTGGCACCCCGGGAACAGCTTCCCCGCGCGG270.24603608529250956No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT250.227811190085657No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGAGTG230.20958629487880445No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG220.20047384727537818No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT210.19136139967195187No Hit
TGTCACTCTATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC210.19136139967195187No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGCGTG210.19136139967195187No Hit
TGTCATCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC210.19136139967195187No Hit
TGTACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC200.1822489520685256No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCCGGCTTGTCCCCCCAGGTGTGTG190.17313650446509932No Hit
CAAGTGTAAGCCTGGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG180.16402405686167304No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGGGTGTG180.16402405686167304No Hit
CAAGTGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTG170.15491160925824676No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCCTGTCCCCCCAGGTGTGTG170.15491160925824676No Hit
CAAGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT170.15491160925824676No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT170.15491160925824676No Hit
CAAGAGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG160.14579916165482049No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGTG150.1366867140513942No Hit
TGTCACTTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC150.1366867140513942No Hit
CAAGTGTAACCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT140.12757426644796793No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGAAGCGAGTTTGTAGAACCAT140.12757426644796793No Hit
CAAGTGTAAGCCTCGGCAAACAAGGTCTGGCTTGTCCCCCCAGGTGTGTG140.12757426644796793No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTCGTCCCCCCAGGTGTGTG140.12757426644796793No Hit
TGTCACCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC130.11846181884454166No Hit
TGTCACTCTCATCGCTGTCGTGACACACCGGGAACAGCTTCCCCGCGCGG130.11846181884454166No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACGGCTTCCCCGCGCGG130.11846181884454166No Hit
CAAGTGAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT120.10934937124111535No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCTCCCCAGGTGTGTG120.10934937124111535No Hit
TGTCACTCTCATCGCTGTCGTGGCGCACCGGGAACAGCTTCCCCGCGCGG120.10934937124111535No Hit
TGTCACTCCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC120.10934937124111535No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCAGGCTTGTCCCCCCAGGTGTGTG120.10934937124111535No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGAG120.10934937124111535No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGCG120.10934937124111535No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCCTCCCCGCGCGG120.10934937124111535No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCATCCCCGCGCGG110.10023692363768909No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCGGGTGTGTG110.10023692363768909No Hit
CAAGGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT110.10023692363768909No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGACTTGTCCCCCCAGGTGTGTG110.10023692363768909No Hit
CAAGTGTAAGCCTCCGAACCACATGACCCACGAGTTTGTAGAACCATGTC110.10023692363768909No Hit
CAAGTGTAAGCCTCGGCAAGCAAGGCCTGGCTTGTCCCCCCAGGTGTGTG110.10023692363768909No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)