FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008127691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008127691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9970
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG516951.84553660982949No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG239023.971915747241727No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT790.7923771313941825No Hit
TGTCCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG590.5917753259779338No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT560.5616850551654965No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAACTTCCCCGCGCGG530.5315947843530592No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT510.5115346038114343No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT470.47141424272818455No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT390.39117352056168503No Hit
CAAGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT270.2708124373119358No Hit
TGTCACTCTCATCGCTGTCGTGGCACCCCGGGAACAGCTTCCCCGCGCGG260.2607823470411234No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT240.2407221664994985No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGCCCCCCCAGGTGTGTG240.2407221664994985No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGTG220.22066198595787362No Hit
CAAGTGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGT210.21063189568706117No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG210.21063189568706117No Hit
CAAGTGTAAGCCTCCGAACCACATGACCCACGAGTTTGTAGAACCATGTC200.20060180541624875No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGAG190.1905717151454363No Hit
TGTCACTCTCATCCCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG180.18054162487462386No Hit
CAAGTGTAAGCCTCGGCAAACGAGGCCTGGCTTGTCCCCCCAGGTGTGTG160.160481444332999No Hit
TGTACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC160.160481444332999No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGAGTG150.15045135406218654No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCCGGCTTGTCCCCCCAGGTGTGTG140.14042126379137412No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTCGTCCCCCCAGGTGTGTG140.14042126379137412No Hit
TGTCACTCTCATCGCTGTCGTGGCACGCCGGGAACAGCTTCCCCGCGCGG140.14042126379137412No Hit
TTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC130.1303911735205617No Hit
TGTCACTCTCAACGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG130.1303911735205617No Hit
TGTCACTTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC130.1303911735205617No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT130.1303911735205617No Hit
TGTCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC120.12036108324974926No Hit
TGTCATCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC120.12036108324974926No Hit
CAAGTGAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT110.11033099297893681No Hit
CAAGTGTAAGCCGCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG110.11033099297893681No Hit
TGTCACTCTCATCGCTGTCGTGGCGCACCGGGAACAGCTTCCCCGCGCGG110.11033099297893681No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT110.11033099297893681No Hit
CAAGTGTAAGCCTCGACAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG110.11033099297893681No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCGGGTGTGTG100.10030090270812438No Hit
TGTCACTCTCACGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC100.10030090270812438No Hit
TGTCACTCTCTCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC100.10030090270812438No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCCTGTCCCCCCAGGTGTGTG100.10030090270812438No Hit
TGTCACTCTATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC100.10030090270812438No Hit
TGTCACTCTCATCGCTGTCGTGGCCCACCGGGAACAGCTTCCCCGCGCGG100.10030090270812438No Hit
CAAGTGTAAGCCACGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG100.10030090270812438No Hit

[OK]Adapter Content

Adapter graph