FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008127924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008127924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10550
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG825278.218009478673No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA7877.459715639810427No Hit
CTTCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA830.7867298578199053No Hit
CTTCCAGGCCTCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG740.7014218009478673No Hit
CTTCCAGGCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA500.47393364928909953No Hit
CTTCCAGGCCTCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA500.47393364928909953No Hit
CTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA460.43601895734597157No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA420.3981042654028436No Hit
CTTCCAGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA360.3412322274881517No Hit
CTTCCAGGCCTCCTGATCCCCACTTCCATAGAGCTTTGCCGACACAGCCA330.3127962085308057No Hit
CTTCCGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA280.2654028436018957No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCCCCTCCTCCGAGCTCCCAGCAGGG270.2559241706161137No Hit
CATCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG260.24644549763033177No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCCCCCAGCAGGG230.21800947867298578No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCCCCTCCGAGCTCCCAGCAGGG210.1990521327014218No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCGGGG200.18957345971563982No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGGGCTCCCAGCAGGG190.18009478672985782No Hit
CTTCCAGGCCTCCTATGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA180.17061611374407584No Hit
CTTCCAGGCCTCCTAGTGCTCTGGGCTCCTCCTCCGAGCTCCCAGCAGGG180.17061611374407584No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCCCCGAGCTCCCAGCAGGG170.16113744075829384No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAACTCCCAGCAGGG160.15165876777251183No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGTTCCTCCTCCGAGCTCCCAGCAGGG160.15165876777251183No Hit
CTTCCAGGCCTCCTAGTGCCCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG150.14218009478672985No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCGGCAGGG150.14218009478672985No Hit
CTTCCAGGCCTCCTAGTGCTCTAGACTCCTCCTCCGAGCTCCCAGCAGGG150.14218009478672985No Hit
CTTCCAGGCCTCCTGGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG140.13270142180094785No Hit
CTTCCAGGCCCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA130.12322274881516589No Hit
TGGGTCATGTGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG130.12322274881516589No Hit
CTTCCAGGCCTCCTAGTGCTCTAAGCTCCTCCTCCGAGCTCCCAGCAGGG110.1042654028436019No Hit
CTTCCAGGCCTCCTAGTGCTCCAGGCTCCTCCTCCGAGCTCCCAGCAGGG110.1042654028436019No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGA110.1042654028436019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)