FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008128137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008128137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Total Bases3.3 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGCAATGAGGAGAAAGAGTCAAGAGAAATGAAGACAA28.695652173913043No Hit
TGTCACTCTCATCGCTGTCGCTCCTTGACCTTGGGCTTCTCAGCAACTCT14.3478260869565215No Hit
CAAGTGTAAGCCTCGGAGGCCTGGAAGTTGAGGTGTAGAACCATGTCGTC14.3478260869565215No Hit
CAAGTGTAAGCCTCCGAACCACATGACCCATTGAGGTGTAGAACCATGTC14.3478260869565215No Hit
TGTCACTCTCATCGCTGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA14.3478260869565215Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAAGTGTAAGCCTGGGCAGCGTGGTGAGACCCCGTCTCTACAAAAAAAAA14.3478260869565215No Hit
TGTCACTCTACGCTGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA14.3478260869565215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAGTGTAAGCCTCGGCTGCAAGTGGCCTCTCCTCCCCGCGGTTGTAGTT14.3478260869565215No Hit
TGTCACTCTCATCGCAGAGTACATGGAAGTAGTGGTATCAACGCAGAGTA14.3478260869565215Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAAGTGTAAGCTCGGCAACAAGAGTGAAAACTCCATCTCAAAAATAAAAA14.3478260869565215No Hit
CAAGTGTAAGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCA14.3478260869565215No Hit
CAAGTGTAAGCCTCGGCCTCCCAAAGTGCCAGGATTACAGGCATGAGCCA14.3478260869565215No Hit
TGTACTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAT14.3478260869565215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTCACTCTCATCGCTGAGTACATGGAAGCAGTGGTATCAACGCCGAGTA14.3478260869565215Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGTCACTCTCATCGCAGAGTACGTGGAAGCAGTGGTATCAACGCAGAGTA14.3478260869565215Clontech Universal Primer Mix Long (96% over 25bp)
TGTCACTCGCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTC14.3478260869565215Clontech Universal Primer Mix Long (96% over 25bp)
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT14.3478260869565215No Hit
CAAGTGAAGCCTCGGCAAAAGGAGAGAGAGAAGACCCATCACGACAAACG14.3478260869565215No Hit
TGTCACTCTCATCGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT14.3478260869565215Clontech Universal Primer Mix Long (100% over 23bp)
CAAGTGTAAGCCTCGGCAAATGCCGCCCAGTCCAGCCTGGACTGCGGGCG14.3478260869565215No Hit
GTCACTCTCATCGCTGTCGGAGGCCGACCATAGGCGCCAGCGCCGGAGAC14.3478260869565215No Hit
TGTCACTCTCATCGCTGTCGAAGCAGCTGTCCAGCTTGCTCAGCAGGATG14.3478260869565215No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)