FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008128171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008128171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12276
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG948577.26458129683937No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT1341.0915607689801239No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT1170.9530791788856305No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT1070.8716194200065167No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT870.7086999022482893No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT730.5946562398175301No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT540.43988269794721413No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGCCCCCCCAGGTGTGTG470.3828608667318345No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT410.33398501140436626No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT410.33398501140436626No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGCGTG360.2932551319648094No Hit
CAAGGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT330.2688172043010753No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGAG320.2606712284131639No Hit
CAAGTGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTG310.25252525252525254No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGAGTG310.25252525252525254No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTCGTCCCCCCAGGTGTGTG310.25252525252525254No Hit
CAAGTGAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT300.24437927663734116No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCCTGTCCCCCCAGGTGTGTG290.2362333007494298No Hit
CAAGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT290.2362333007494298No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGTG260.21179537308569565No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCGGGTGTGTG250.20364939719778433No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCCGGCTTGTCCCCCCAGGTGTGTG240.19550342130987292No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGCG240.19550342130987292No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG230.18735744542196156No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGTTTGTCCCCCCAGGTGTGTG190.15477354187031606No Hit
CAAGTGTAAGCCACGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG190.15477354187031606No Hit
CCAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGT180.1466275659824047No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGACCCCCCAGGTGTGTG170.13848159009449332No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGACTTGTCCCCCCAGGTGTGTG170.13848159009449332No Hit
CAAGTGTAACCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT160.13033561420658196No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTATCCCCCCAGGTGTGTG160.13033561420658196No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCTCCCAGGTGTGTG160.13033561420658196No Hit
CAAGTGTAAGCCTCGGCAAACGAGGCCTGGCTTGTCCCCCCAGGTGTGTG160.13033561420658196No Hit
CAAGTGTAAGCCTGGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG150.12218963831867058No Hit
CAAGTGTAAGCCTCGGTAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG150.12218963831867058No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTTCCCCCAGGTGTGTG150.12218963831867058No Hit
CAAGTGTAAGCCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG140.11404366243075921No Hit
CAAGTGTAAGCATCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG140.11404366243075921No Hit
CAAGTGTAAGCCTCGGCAAGCAAGGCCTGGCTTGTCCCCCCAGGTGTGTG140.11404366243075921No Hit
CAAGTGTACGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG130.10589768654284783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)