FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008128253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008128253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10495
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG470044.78323010957599No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG326031.062410671748452No Hit
TGTCCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG790.7527393997141496No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT620.5907575035731301No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT570.5431157694140066No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT520.4954740352548833No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT520.4954740352548833No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT340.32396379228203903No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGCCCCCCCAGGTGTGTG320.3049070986183897No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG270.25726536445926634No Hit
GTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC260.24773701762744166No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT250.23820867079561697No Hit
TGTCACTCTCATCGCTGTCGTGGCACCCCGGGAACAGCTTCCCCGCGCGG240.2286803239637923No Hit
TGTCACTTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC240.2286803239637923No Hit
TGTCACTCTCATCCCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG240.2286803239637923No Hit
TGTACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC230.2191519771319676No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTCGTCCCCCCAGGTGTGTG220.20962363030014292No Hit
TTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC210.20009528346831826No Hit
TGTCACTCTATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC210.20009528346831826No Hit
CAAGTGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTG200.19056693663649357No Hit
CAAGTGTAAGCCTCGGCAAACGAGGCCTGGCTTGTCCCCCCAGGTGTGTG200.19056693663649357No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCGGGTGTGTG190.1810385898046689No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT190.1810385898046689No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT180.1715102429728442No Hit
CAAGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT170.16198189614101952No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGTG160.15245354930919486No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGCGTG160.15245354930919486No Hit
CAAGGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT150.14292520247737017No Hit
TGTCACTCTCATCGCTGTCGTGGTACACCGGGAACAGCTTCCCCGCGCGG150.14292520247737017No Hit
CAAGTGTAAGCCTCGGCAAGCAAGGCCTGGCTTGTCCCCCCAGGTGTGTG150.14292520247737017No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGAGTG140.1333968556455455No Hit
TGTCACTCTCATCGCTGTCGTGGCCCACCGGGAACAGCTTCCCCGCGCGG140.1333968556455455No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACGGCTTCCCCGCGCGG140.1333968556455455No Hit
TGTCACTCTCATCGCTGTCGTGGCACGCCGGGAACAGCTTCCCCGCGCGG140.1333968556455455No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTCCCCCGCGCGG130.12386850881372083No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCCGGCTTGTCCCCCCAGGTGTGTG130.12386850881372083No Hit
CAAGTGAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT130.12386850881372083No Hit
TGTCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC130.12386850881372083No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGAG130.12386850881372083No Hit
TGTCATCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC130.12386850881372083No Hit
TGTCACTCTCTCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC120.11434016198189614No Hit
CAAGTGTAAGCCTCGGCAAACAAGGTCTGGCTTGTCCCCCCAGGTGTGTG120.11434016198189614No Hit
TGTCACTCTCACGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC110.10481181515007146No Hit
CAAGTGTAACCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT110.10481181515007146No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCAGGCTTGTCCCCCCAGGTGTGTG110.10481181515007146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)