FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008128307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008128307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154
Total Bases167.2 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG87275.5632582322357No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT151.2998266897746966No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT141.2131715771230502No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT90.779896013864818No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT80.6932409012131715No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT70.6065857885615251No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT60.5199306759098787No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCCTGTCCCCCCAGGTGTGTG50.43327556325823224No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT40.34662045060658575No Hit
CAAGTGTAAGCCTCGGCAAGCAAGGCCTGGCTTGTCCCCCCAGGTGTGTG40.34662045060658575No Hit
CAAGTGAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT40.34662045060658575No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCTTGGCTTGTCCCCCCAGGTGTGTG40.34662045060658575No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCGGGTGTGTG30.25996533795493937No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTAGCTTGTCCCCCCAGGTGTGTG30.25996533795493937No Hit
CAAGGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT30.25996533795493937No Hit
CAAGTGTAACCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT30.25996533795493937No Hit
CAAGAGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG30.25996533795493937No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCCGGCTTGTCCCCCCAGGTGTGTG30.25996533795493937No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGACTTGTCCCCCCAGGTGTGTG30.25996533795493937No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGCCCCCCCAGGTGTGTG30.25996533795493937No Hit
CAAGTGTAAACCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG30.25996533795493937No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT30.25996533795493937No Hit
CAAGTGTAAGCCTCGGCAAACGAGGCCTGGCTTGTCCCCCCAGGTGTGTG30.25996533795493937No Hit
CAAGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT30.25996533795493937No Hit
CAAGTGTAAGCCACGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG20.17331022530329288No Hit
CAAGTGTAAGCCTCGGCAAACAAGGACTGGCTTGTCCCCCCAGGTGTGTG20.17331022530329288No Hit
TGTCACTCTCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA20.17331022530329288Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAAGTGTAAGCCGCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG20.17331022530329288No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCCGGTGTGTG20.17331022530329288No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTTTGTG20.17331022530329288No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTTCCCCCAGGTGTGTG20.17331022530329288No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGCGTG20.17331022530329288No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGTG20.17331022530329288No Hit
CAAGTGTAAGCCTCGGCAAAGACGCACAGTCCCCACAGTGCCCCAGGGGC20.17331022530329288No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGAGTG20.17331022530329288No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGCG20.17331022530329288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)