FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008128337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008128337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141
Total Bases20.4 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG8560.28368794326241No Hit
CAAGTGTAAGCCTCGGCAAAGACGCACAGTCCCCACAGTGCCCCAGGGGC42.8368794326241136No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT42.8368794326241136No Hit
CAAGTGTAAGCCTCGGCAGCAGATTCTCCTAATTTTTAACACTAAAATTC21.4184397163120568No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT21.4184397163120568No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT21.4184397163120568No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT21.4184397163120568No Hit
CAAGTGTAAGCCTCGGCAACATAGGGAGACCCCGTCTCTACAAAAAAATC21.4184397163120568No Hit
CAAGTGTAAGCCTCCGAACCACATGACCCATTGAGGTGTAGAACCATGTC10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCTCCCCAGGTGTGTG10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCGTGTCCCCCCAGGTGTGTG10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCAAACCTTTGCAGGGAACATTCAGAGTTCGGATT10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCA10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCAGGCTTGTCCCCCCAGGTGTGTG10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCAAACACGGCCTGGCTTGTCCCCCCAGGTGTGAG10.7092198581560284No Hit
TGTCAGTCTCATCGCTGTATGCTGTGTGGAAGGTCGAATCATCATCATCA10.7092198581560284No Hit
AAAGTGTAAGCCTCGGCAAAACTTTTCAGGGAACATTAAGAGTTAGGATT10.7092198581560284No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGACTTGTCCCCCCAGGTGTGTGT10.7092198581560284No Hit
CAAGTGTAGGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.7092198581560284No Hit
TGTCACTCTCATCGCTGTCGTCAGTGTAGCACTAGGAGGCCTGGAAGTTG10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTAGTCCCCCCAGGAGTGTG10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCCTCCCAAGCAGCTGGGACTGCAGGCATACAACC10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTTGCTTGTCCCCCCAGGTGTGTG10.7092198581560284No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTAGCTTGTCCCCCCAGGTGTGTGT10.7092198581560284No Hit
TGTCACTCTCATCGCTGTGCTAAAACATAACTTGAACATCGTGATTAAAC10.7092198581560284No Hit
CAAGTGTAAGACGCGGCNATCATGGCCAGGATTGTCCCCCCAGGAGTGAG10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCAAACAATGCCTGGCTTGTCCCCCCAGGTGTGTG10.7092198581560284No Hit
CAATGTAAGCCTCGGCAACATGGTGAGACCCTGTCTCTACAAAAAAAATA10.7092198581560284No Hit
CAAGTGTAAGGGTGCGGTTTGTACCCAATTATGGTCTTTTATTTTTTTGG10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCAAACGAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.7092198581560284No Hit
CAAGTGTAAGCCGCGGCAATCATGGCCTGGCTTGTCCCCCCAGGTGTGTG10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCAACACAGCAGGACTGTGATTTTCTTTGGAGACT10.7092198581560284No Hit
CAACACATACCCACGGCAGCTCGGCCCCCCCCCGGACCTTAGATCCCCCC10.7092198581560284No Hit
CAAGTGTAAGCCTCGGTAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG10.7092198581560284No Hit
CAAGTGTAAGCCTCGGAAAACAAGGCCTGGCTTGTACCCCCAGGTGAGTG10.7092198581560284No Hit
TGTCACTCTCATCGCCGAACCACATGACCCATTGAGGTGTAGAACCATGT10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGAG10.7092198581560284No Hit
CATGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCACCAGGTGTGTG10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCGTGCTTGTCGCCCCAGGTGTGTG10.7092198581560284No Hit
CAACTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.7092198581560284No Hit
CAAGTGTAAGCAGTGGTATCAACGCAGAGTACATGGGAGGCAGTGGTATC10.7092198581560284Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAGTGTAAGCCTCGGCAAAAAAGGACTGGCTTGTCCCCACAGGTGTGTG10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCAGCAGTCAATCCTCCAGAACCTATGCTGGTGGA10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCAAACAAGGTCTGGCTTGTCCCCCCAGGTGTGTG10.7092198581560284No Hit
CAAGTGTAAGCCTCGGCATCTGCGGGCGCCTTGCAAGGAACACCCGGCAG10.7092198581560284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)