FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008128483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008128483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6
Total Bases868 bp
Sequences flagged as poor quality0
Sequence length143-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACTCTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA116.666666666666664Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGTCACTCTCATCGCTGTCGTCAGTGTAGCATAGGAGGCCTGGAAGTTGA116.666666666666664No Hit
CAAGTGTAAGCCTCGGAGGCCTGGAAGTTGAGGTGTAGAACCATGTCGTC116.666666666666664No Hit
CAAGTGTAAGCCTCCGAACCACATGACCCATTGAGGTGTAGAACCATGTC116.666666666666664No Hit
TGTCACTCTCATCGCTGTCGTCAGTGTAGCACTAGGAGGCCTGGAAGTTG116.666666666666664No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCAGGCTTGTCCCCCCAGGAGTGTG116.666666666666664No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)