FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008128628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008128628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12947
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG593545.84073530547617No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA493138.086043098787364No Hit
TGGGTCTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG1030.7955510929172781No Hit
TGGGTATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG890.6874179346566772No Hit
TGGGTCATTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG840.6487989495636054No Hit
TGGGTCATGTGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG690.5329419942843903No Hit
TGGGTCAGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG610.4711516181354754No Hit
CTTCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA540.41708503900517496No Hit
CTTCCAGGCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA420.3243994747818027No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGTG380.29350428670734535No Hit
TGGGTCATGGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG360.2780566926701166No Hit
TGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG350.27033289565150226No Hit
CTTCCAGGCCTCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG350.27033289565150226No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA300.23171391055843055No Hit
CTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA270.20854251950258748No Hit
CTTCCAGGCCTCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA270.20854251950258748No Hit
CTTCCGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA250.19309492546535878No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCCCCTCCTCCGAGCTCCCAGCAGGG250.19309492546535878No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGCGTGTTGAGAA240.18537112844674442No Hit
TGGGTCGTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA230.17764733142813005No Hit
CTTCCAGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA220.16992353440951571No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGAGGTGTGTGTTGAGAA220.16992353440951571No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCCCCTCCGAGCTCCCAGCAGGG210.16219973739090135No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCCCCCAGCAGGG210.16219973739090135No Hit
TGGGCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG200.154475940372287No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGCGTGTGTTGAGAA180.1390283463350583No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGGGAA170.13130454931644397No Hit
TGGGTCATGTGGTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG160.12358075229782961No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCCCAGTGGTGTGTGTTGAGAA160.12358075229782961No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAG150.11585695527921527No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATCCTCAGTGGTGTGTGTTGAGAA140.10813315826060091No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGCGTTGAGAA130.10040936124198656No Hit
CTTCCAGGCCTCCTAGTGCTCTGGGCTCCTCCTCCGAGCTCCCAGCAGGG130.10040936124198656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)