FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008128654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008128654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10215
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG629361.6054821341165No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA236923.191385217816936No Hit
CTTCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA690.6754772393538914No Hit
TGGGTCTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG680.6656877141458639No Hit
TGGGTATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG580.5677924620655899No Hit
CTTCCAGGCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA520.5090553108174254No Hit
TGGGTCATTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG470.4601076847772883No Hit
CTTCCAGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA410.4013705335291239No Hit
CTTCCAGGCCTCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG380.3720019579050416No Hit
TGGGTCAGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG370.3622124326970142No Hit
CTTCCAGGCCTCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA350.34263338228095935No Hit
TGGGTCATGTGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG350.34263338228095935No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA280.27410670582476754No Hit
CTTCCGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA250.24473813020068524No Hit
CTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA240.23494860499265785No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCCCCTCCGAGCTCCCAGCAGGG210.2055800293685756No Hit
CTTCCAGGCCTCCTGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA190.1860009789525208No Hit
CATCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG160.15663240332843856No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGA160.15663240332843856No Hit
TGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG150.14684287812041116No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGTTCCTCCTCCGAGCTCCCAGCAGGG150.14684287812041116No Hit
CTTCCAGGCCCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA150.14684287812041116No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCCCCTCCTCCGAGCTCCCAGCAGGG150.14684287812041116No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGGGCTCCCAGCAGGG140.13705335291238377No Hit
TGGGTCGTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA140.13705335291238377No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCGGCAGGG130.12726382770435635No Hit
TGGGTCATGGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG120.11747430249632893No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCGGGG120.11747430249632893No Hit
TGGGCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG120.11747430249632893No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCCCCGAGCTCCCAGCAGGG120.11747430249632893No Hit
CTTCCAGGCCTCCTAGTGCCCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG110.1076847772883015No Hit
CTTCCAGGCCTCCTATGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA110.1076847772883015No Hit
CTTCCAGGCCTCCTAGCGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG110.1076847772883015No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)