FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008128714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008128714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8432
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG429550.936907020872866No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA272132.26992409867172No Hit
TGGGTCTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG901.0673624288425048No Hit
TGGGTATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG740.8776091081593927No Hit
TGGGTCATTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG480.5692599620493358No Hit
TGGGTCAGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG440.5218216318785579No Hit
TGGGTCATGTGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG420.4981024667931689No Hit
CTTCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA400.47438330170777987No Hit
CTTCCAGGCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA310.3676470588235294No Hit
CTTCCGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA310.3676470588235294No Hit
CTTCCAGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA270.3202087286527514No Hit
CTTCCAGGCCTCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG270.3202087286527514No Hit
TGGGTCATGGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG260.3083491461100569No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGAGGTGTGTGTTGAGAA200.23719165085388993No Hit
TGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG200.23719165085388993No Hit
TGGGTCGTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA200.23719165085388993No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA190.22533206831119545No Hit
CTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA170.20161290322580644No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCCCCTCCTCCGAGCTCCCAGCAGGG170.20161290322580644No Hit
CTTCCAGGCCTCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA130.15417457305502846No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCCCCTCCGAGCTCCCAGCAGGG120.14231499051233396No Hit
CATCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG110.13045540796963948No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGTAGGG110.13045540796963948No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCCCCCAGCAGGG110.13045540796963948No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCGGGG110.13045540796963948No Hit
TGGGCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG110.13045540796963948No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGCGTGTTGAGAA110.13045540796963948No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGGGAA110.13045540796963948No Hit
CTTCCAGGCCTCCTAGTGCCCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG100.11859582542694497No Hit
TGGGTCATGTGGTTCGGAGACGACAAATTCTCAGTGGTGTGTGTTGAGAA90.10673624288425047No Hit
CTTCCAGGCCTCCTATGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA90.10673624288425047No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAACTCCCAGCAGGG90.10673624288425047No Hit
CTTCCAGGCCTCCTAGTGCTCTGGGCTCCTCCTCCGAGCTCCCAGCAGGG90.10673624288425047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)