FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008128844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008128844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11624
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG819170.46627666896077No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA175615.106675843083275No Hit
CTTCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA1030.8860977288368892No Hit
CTTCCAGGCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA590.5075705437026841No Hit
TGGGTCTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG530.4559532002752925No Hit
CTTCCAGGCCTCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA490.4215416379903647No Hit
CTTCCAGGCCTCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG470.4043358568479009No Hit
CTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA450.38713007570543706No Hit
TGGGTATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG430.36992429456297315No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA340.2924982794218857No Hit
CTTCCAGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA330.28389538885065385No Hit
TGGGTCATTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG320.27529249827942187No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCCCCTCCGAGCTCCCAGCAGGG310.26668960770818995No Hit
CTTCCGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA310.26668960770818995No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCCCCCAGCAGGG260.2236751548520303No Hit
TGGGTCAGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG260.2236751548520303No Hit
TGGGTCATGGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG210.18066070199587062No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCGGGG210.18066070199587062No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCCCCTCCTCCGAGCTCCCAGCAGGG200.1720578114246387No Hit
CTTCCAGGCCTCCTAGTGCTCTAGACTCCTCCTCCGAGCTCCCAGCAGGG190.16345492085340674No Hit
CTTCCAGGCCTCCTAGTGCTCTGGGCTCCTCCTCCGAGCTCCCAGCAGGG180.1548520302821748No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCCCCGAGCTCCCAGCAGGG180.1548520302821748No Hit
TGGGTCATGTGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG180.1548520302821748No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAACTCCCAGCAGGG170.14624913971094286No Hit
CATCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG170.14624913971094286No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCGGCAGGG150.129043358568479No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGTTCCTCCTCCGAGCTCCCAGCAGGG150.129043358568479No Hit
TGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG140.12044046799724707No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGTTCCCAGCAGGG140.12044046799724707No Hit
CTTCCAGGCCTCCTAGTGCTCTAAGCTCCTCCTCCGAGCTCCCAGCAGGG130.11183757742601515No Hit
CTTCCAGGCCTCCTAGTGCTCCAGGCTCCTCCTCCGAGCTCCCAGCAGGG130.11183757742601515No Hit
CCTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG120.10323468685478321No Hit
CTTCCAGGCCTCCTATGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA120.10323468685478321No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGGGCTCCCAGCAGGG120.10323468685478321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)