FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008128963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008128963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Total Bases1.8 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG215.384615384615385No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG17.6923076923076925No Hit
TGTCACTCTCATCGCTGTCGGCCAACCAGAGCACCCGCAGCACAGAGCCA17.6923076923076925No Hit
TGTCACTCTCATCGCTGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA17.6923076923076925Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGTCACTCTCATCGCTGTCGGGGCACACCGGGAACAGCTGCCCCGCGCGG17.6923076923076925No Hit
TGTCACTTAACGCAGAGTACATGGAAGCAGTTGTATCAACGCTGAGTAAA17.6923076923076925No Hit
TGTCACTCTCATCGCAGAGTACATGGAAGCAGTGGTTTCAACGCAGAGTA17.6923076923076925Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAAGAGTAAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGAATCAAC17.6923076923076925Clontech SMARTer II A Oligonucleotide (95% over 22bp)
TGTCATCCACGCAGAGTACATGGAAGCAGTGGTATCGACGCAGAGTACAT17.6923076923076925Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAAGTGTAAGCCTCGGCAAACAAGGCCTGTCTTGCCCCCCCAGGTGTGTG17.6923076923076925No Hit
CAAGTGTAAGCCTAGGAGGCCTGGAAGCAGTCATGTAGAACCATGTCGTC17.6923076923076925No Hit
CAAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTGAG17.6923076923076925Clontech Universal Primer Mix Long (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)