FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13543
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG601344.39932068227128No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA532639.326589381968546No Hit
TGGGTCTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG1310.9672893745846564No Hit
TGGGTATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG1030.7605405006276305No Hit
TGGGTCAGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG790.583327180093037No Hit
CTTCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA740.5464077383149967No Hit
TGGGTCATGTGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG740.5464077383149967No Hit
TGGGTCATTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG620.4578010780476999No Hit
CTTCCAGGCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA480.35442664106918703No Hit
TGGGTCATGGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG470.347042752713579No Hit
TGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG380.2805877575131064No Hit
CTTCCAGGCCTCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG360.2658199808018903No Hit
CTTCCAGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA320.236284427379458No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA320.236284427379458No Hit
CTTCCAGGCCTCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA300.2215166506682419No Hit
CTTCCGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA250.18459720889020156No Hit
TGGGTCGTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA230.16982943217898544No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGCGTGTTGAGAA230.16982943217898544No Hit
TGGGCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG220.1624455438233774No Hit
TGGGTCATGTGGTTCGGAGACGACAAATTCTCAGTGGTGTGTGTTGAGAA210.15506165546776932No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCACAGCAGGG200.14767776711216127No Hit
CTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA190.1402938787565532No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCCCCTCCTCCGAGCTCCCAGCAGGG190.1402938787565532No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCGGTGGTGTGTGTTGAGAA180.13290999040094514No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATCCTCAGTGGTGTGTGTTGAGAA170.12552610204533707No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGAGGTGTGTGTTGAGAA170.12552610204533707No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGCGTGTGTTGAGAA160.118142213689729No Hit
TGGGTCATGTGGTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG160.118142213689729No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCCCCGAGCTCCCAGCAGGG150.11075832533412094No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCCCAGTGGTGTGTGTTGAGAA150.11075832533412094No Hit
TGGGTCATGTGGTTCGGAGACAGCAAATTCTCAGTGGTGTGTGTTGAGAA140.10337443697851288No Hit
CATCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG140.10337443697851288No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCCCCTCCGAGCTCCCAGCAGGG140.10337443697851288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)