FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11575
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG488342.18574514038877No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG391133.78833693304536No Hit
TGTCCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG1261.0885529157667386No Hit
TGTCACTCTAATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG990.8552915766738661No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT770.6652267818574513No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT470.4060475161987041No Hit
CAAGTGTAAGCCTCTGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG390.33693304535637153No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT380.3282937365010799No Hit
TGTCACTCTATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC370.31965442764578833No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT330.28509719222462204No Hit
TGTCACTTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC310.26781857451403884No Hit
TGTCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC300.2591792656587473No Hit
TGTACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC290.2505399568034557No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT290.2505399568034557No Hit
TGTCACTCTCATCGCTGTCGTGGCACCCCGGGAACAGCTTCCCCGCGCGG280.24190064794816415No Hit
TGTCACTCTCATCCCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG280.24190064794816415No Hit
TTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC260.22462203023758098No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCACGCGG230.19870410367170627No Hit
TGTCACTCTCATCGCTGTCGTGGCACGCCGGGAACAGCTTCCCCGCGCGG210.18142548596112312No Hit
TGTCACTCTCACGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC180.15550755939524838No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG180.15550755939524838No Hit
TGTCATCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC180.15550755939524838No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGCCCCCCCAGGTGTGTG170.1468682505399568No Hit
CAAGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT170.1468682505399568No Hit
TGTCACTCTCATCGCTGTCGTGACACACCGGGAACAGCTTCCCCGCGCGG170.1468682505399568No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGGACAGCTTCCCCGCGCGG150.12958963282937366No Hit
TGTCACTCTCATGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC150.12958963282937366No Hit
TGTCACCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC140.12095032397408208No Hit
TGTCACTCCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC140.12095032397408208No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT140.12095032397408208No Hit
TGCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC140.12095032397408208No Hit
TGTCACTCTCATCGCTGTCGTGGCCCACCGGGAACAGCTTCCCCGCGCGG140.12095032397408208No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGCGTG140.12095032397408208No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT130.11231101511879049No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAACTTCCCCGCGCGG130.11231101511879049No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACGGCTTCCCCGCGCGG130.11231101511879049No Hit
CAAGTGTAACCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT120.10367170626349892No Hit
CAAGTGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTG120.10367170626349892No Hit
TGTCACTCTCATCGCTGTCGTGGCGCACCGGGAACAGCTTCCCCGCGCGG120.10367170626349892No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGTTTCCCCGCGCGG120.10367170626349892No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAGCAGCTTCCCCGCGCGG120.10367170626349892No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGA120.10367170626349892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)