FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8195
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG696685.00305064063454No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA1001.2202562538133008No Hit
CTTCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA971.1836485661989018No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA570.6955460646735814No Hit
CTTCCAGGCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA550.6711409395973155No Hit
CTTCCAGGCCTCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG450.5491153142159854No Hit
CTTCCAGGCCTCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA350.4270896888346553No Hit
CTTCCAGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA310.37827943868212327No Hit
CTTCCGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA310.37827943868212327No Hit
CTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA250.3050640634533252No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAACTCCCAGCAGGG220.2684563758389262No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCCCCTCCTCCGAGCTCCCAGCAGGG220.2684563758389262No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCCCCCAGCAGGG210.25625381330079317No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCCCCTCCGAGCTCCCAGCAGGG170.20744356314826112No Hit
CATCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG160.19524100061012814No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGTTCCCAGCAGGG140.17083587553386212No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCTGAGCTCCCAGCAGGG130.1586333129957291No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCGGGG130.1586333129957291No Hit
CTTCCAGGCCTCCTAGTGCCCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG120.1464307504575961No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCTAGCAGGG120.1464307504575961No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCCCCGAGCTCCCAGCAGGG120.1464307504575961No Hit
TTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG120.1464307504575961No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGTTCCTCCTCCGAGCTCCCAGCAGGG120.1464307504575961No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCGGCAGGG120.1464307504575961No Hit
CTTCCAGGCCTCCTTGACCTTGGGCTTCTCTGCGTTGATACCACTGCTTC110.1342281879194631Clontech SMART CDS Primer II A (95% over 24bp)
CTTCCAGGCCTCCTAGTGCTCTAGGCTCTTCCTCCGAGCTCCCAGCAGGG110.1342281879194631No Hit
CTTCCAGGCCTCCTAGTGCTCTGGGCTCCTCCTCCGAGCTCCCAGCAGGG110.1342281879194631No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGAG110.1342281879194631No Hit
CTTCCAGGCCTCCTAGTGCTCCAGGCTCCTCCTCCGAGCTCCCAGCAGGG110.1342281879194631No Hit
CTTCCAGGCCTCCTGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA100.12202562538133008No Hit
CTTCCAGGCCTCCTATGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA100.12202562538133008No Hit
CTTCCAGGCCTCCTAGCGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG90.10982306284319707No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAACAGGG90.10982306284319707No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGTAGGG90.10982306284319707No Hit
TGGGTCATGTGGTTCGACAGCGATGAGAGTGACACTGTTCAGACCAAGTC90.10982306284319707No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGGGCTCCCAGCAGGG90.10982306284319707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)