FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402
Total Bases928.2 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG372058.10684161199625No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA164725.726335520149952No Hit
CTTCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA460.718525460793502No Hit
CTTCCAGGCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA370.577944392377382No Hit
TGGGTCATGTGGTTCGACAGCGATGAGAGTGACACTGTTCAGACCAAGTC330.5154639175257731No Hit
CTTCCAGGCCTCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG260.4061230865354577No Hit
TGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG240.37488284910965325No Hit
TGGGTCATGTGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG220.3436426116838488No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA220.3436426116838488No Hit
CTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA210.32802249297094654No Hit
CTTCCAGGCCTCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA210.32802249297094654No Hit
TGGGTCATGTGGTTGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG160.24992189940643547No Hit
CTTCCAGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA150.23430178069353325No Hit
TGGGTCATGTGGTCCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA130.20306154326772885No Hit
CTTCCGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA130.20306154326772885No Hit
TGGGTCATTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG120.18744142455482662No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCCCCCAGCAGGG120.18744142455482662No Hit
TTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG110.1718213058419244No Hit
TGGGTATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG110.1718213058419244No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCGGGG110.1718213058419244No Hit
TGGGTCTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG110.1718213058419244No Hit
CTTCCAGGCCTCCTAGTGCCCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG100.1562011871290222No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCCCCTCCGAGCTCCCAGCAGGG100.1562011871290222No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCCCCTCCTCCGAGCTCCCAGCAGGG100.1562011871290222No Hit
CATCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG90.14058106841611998No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGAGGTGTGTGTTGAGAA90.14058106841611998No Hit
TGGGTCATGGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG90.14058106841611998No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAACTCCCAGCAGGG90.14058106841611998No Hit
CTTCCAGGCCTCCTTGACCTTGGGCTTCTCTGCGTTGATACCACTGCTTC80.12496094970321774Clontech SMART CDS Primer II A (95% over 24bp)
CTTCCAGGCCTCCTGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA80.12496094970321774No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCGGCAGGG80.12496094970321774No Hit
CTTCCAGGCCCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA80.12496094970321774No Hit
CTTCCAGGCCTCCAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA80.12496094970321774No Hit
TGGGTCATGTGGTTGGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA80.12496094970321774No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGGGAA80.12496094970321774No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGTAGGG70.10934083099031554No Hit
CTTCCAGGCCTCCTAGTGCTCTAGACTCCTCCTCCGAGCTCCCAGCAGGG70.10934083099031554No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGGGCTCCCAGCAGGG70.10934083099031554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)