FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722
Total Bases249.6 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA107362.311265969802555No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG23213.472706155632984No Hit
TGGGTCATGTGGTTCGACAGCGATGAGAGTGACACTGTTCAGACCAAGTC221.2775842044134729No Hit
TGGGTCATGTGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG181.0452961672473868No Hit
TGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG160.9291521486643438No Hit
TGGGTCATTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG110.6387921022067364No Hit
TGGGTCATGTGGTTGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG100.5807200929152149No Hit
TGGGTCATGTGGTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG80.4645760743321719No Hit
TGGGTATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG80.4645760743321719No Hit
TGGGTCTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG80.4645760743321719No Hit
CTTCCAGGCCTCCTGATCCCCACTTCCATAGAGCTTTGCCGACACAGCCA70.40650406504065045No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGAGGTGTGTGTTGAGAA50.29036004645760743No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGGGAA50.29036004645760743No Hit
CTTCCAGGCCTCCTTGACCTTGGGCTTCTCTGCGTTGATACCACTGCTTC40.23228803716608595Clontech SMART CDS Primer II A (95% over 24bp)
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAATGGTGTGTGTTGAGAA40.23228803716608595No Hit
CTTCCAGGCCTCCTAGTGCTCCTTTGACATCTGAAACGGTGTAAACTGAA40.23228803716608595No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGCGTGTGTTGAGAA40.23228803716608595No Hit
TGGGTCATGTGGTTCGGAGACGACAAATTCTCAGTGGTGTGTGTTGAGAA40.23228803716608595No Hit
TGGGTCATGTGGTTCGGAGACAGCAAATTCTCAGTGGTGTGTGTTGAGAA40.23228803716608595No Hit
TGGGTCATGGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG40.23228803716608595No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGGA40.23228803716608595No Hit
TGGGTCATGTGGTCCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA40.23228803716608595No Hit
CTTCCAGGCCTCCTTGACCTTGGGCTTCTCGTCCCCCCATGTACTCTGCG40.23228803716608595No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCGGTGGTGTGTGTTGAGAA40.23228803716608595No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATACTCAGTGGTGTGTGTTGAGAA30.17421602787456447No Hit
TGGGTCATGTGGTTCGGAGACGGCAAACTCTCAGTGGTGTGTGTTGAGAA30.17421602787456447No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAG30.17421602787456447No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTATGTGTTGAGAA30.17421602787456447No Hit
TGGGTCATGTGGTTCGGAGACGATCAGATACCGTCGTAGTTCCGACCATA30.17421602787456447No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGAGTTGAGAA30.17421602787456447No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCCCCTCCGAGCTCCCAGCAGGG30.17421602787456447No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAAAA30.17421602787456447No Hit
GGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG30.17421602787456447No Hit
TGGGTCATGTGGTTCGGGGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA30.17421602787456447No Hit
TGGGTCATGTGGTTCGGCAGCGATGAGAGTGACACTGTTCAGACCAAGTC30.17421602787456447No Hit
TGGGCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG30.17421602787456447No Hit
CTTCCAGGCCTCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA30.17421602787456447No Hit
CTTCCAGGCCTCCTAGTGCTGGGATTACAGGCGTGAGCCACTGCTACGGC30.17421602787456447No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGAGTGTGTTGAGAA20.11614401858304298No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGCTGAGAA20.11614401858304298No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCACAGTGGTGTGTGTTGAGAA20.11614401858304298No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATCCTCAGTGGTGTGTGTTGAGAA20.11614401858304298No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTATCAGTGGTGTGTGTTGAGAA20.11614401858304298No Hit
TGGGTCATGAGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA20.11614401858304298No Hit
CTTCCAGGCCTCCTAGACCTTGGGCTTCTCTGCGTTGATACCACTGCTTC20.11614401858304298Clontech SMART CDS Primer II A (95% over 24bp)
TGGGTCTGTGGTTCGGAGACGGCAGATTCTCAGTGGTGTGTGTTGAGAAG20.11614401858304298No Hit
TGGGTCATGTGGTTCGGAGACGGCAAGTTCTCAGTGGTGTGTGTTGAGAA20.11614401858304298No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCGGCAGGG20.11614401858304298No Hit
AGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGAGGTGTGTGTTGAGAA20.11614401858304298No Hit
TGGGTCAGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG20.11614401858304298No Hit
TGGGTCATGTGGTTCGGAGACGGCGAATTCTCAGTGGTGTGTGTTGAGAA20.11614401858304298No Hit
TGGGTCATGCGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA20.11614401858304298No Hit
CTTCCAGGCCTCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG20.11614401858304298No Hit
TGGGTCATGTGGTTGGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA20.11614401858304298No Hit
TGAGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA20.11614401858304298No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTCGAGAA20.11614401858304298No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGCGTGTTGAGAA20.11614401858304298No Hit
CTTCCGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA20.11614401858304298No Hit
CTTCCAGGCCTCCTAGTGCTGGCTGACAGCTTCTCCGGGGCTAGCACACT20.11614401858304298No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA20.11614401858304298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)