FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722
Total Bases247.9 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG95555.45876887340302No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG19911.556329849012775No Hit
TGTCACTCTCATCGCTGTCGAACCACATGACCCATCCAACTGTAGAACCA211.2195121951219512No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGA201.1614401858304297No Hit
TGTCCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG170.9872241579558653No Hit
TGTCATCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC130.7549361207897793No Hit
TGTCACTCTATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC120.6968641114982579No Hit
TGTCACTCTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA110.6387921022067364Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGTCACTTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC110.6387921022067364No Hit
TGTCACTCTCATCGCTGTCGTGACACACCGGGAACAGCTTCCCCGCGCGG90.5226480836236934No Hit
TGTACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC80.4645760743321719No Hit
TGTCACTCTCATCGCTGTTGTGGCACACCGGGAACAGCTTCCCCGCGCGG70.40650406504065045No Hit
CAAGTGTAAGCCTCGGCAAAAAGGCAGCTGGAATCGACGAACAGGAAAAC60.34843205574912894No Hit
TGCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC50.29036004645760743No Hit
TGTCACTCTCATCGCTGTCGTGGCCCACCGGGAACAGCTTCCCCGCGCGG50.29036004645760743No Hit
TGTCACTCTCTCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC50.29036004645760743No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT50.29036004645760743No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGTTTCCCCGCGCGG50.29036004645760743No Hit
TGTCACTCTCATCCCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG50.29036004645760743No Hit
TGTCACCCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG40.23228803716608595No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTCCCCCGCGCGG40.23228803716608595No Hit
TGTCACTCTCATCGCTGTCGCGGCACACCGGGAACAGCTTCCCCGCGCGG40.23228803716608595No Hit
TTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC40.23228803716608595No Hit
TGTCACTCTCATCGCTGTCGGGGCACACCGGGAACAGCTGCCCCGCGCGG40.23228803716608595No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGGACAGCTTCCCCGCGCGG40.23228803716608595No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAGCAGCTTCCCCGCGCGG30.17421602787456447No Hit
TGTCACTCTCACGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC30.17421602787456447No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT30.17421602787456447No Hit
TGTCACTCTCATCGCTGCCGAACCACATGACCCATCCAACTGTAGAACCA30.17421602787456447No Hit
TGTCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC30.17421602787456447No Hit
TGTCACTCTCATAGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG30.17421602787456447No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT30.17421602787456447No Hit
TGTCACTCTCATCGCTTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC30.17421602787456447No Hit
TGTCACTCTCAACGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG30.17421602787456447No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCCTCCCCGCGCGG30.17421602787456447No Hit
TGTCACTCTCATCGCTGTCGTGGCGCACCGGGAACAGCTTCCCCGCGCGG30.17421602787456447No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAACTTCCCCGCGCGG30.17421602787456447No Hit
CAAGTGTAAGCCTCGGCAAACGAGGCCTGGCTTGTCCCCCCAGGTGTGTG30.17421602787456447No Hit
TGTCACTGTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG20.11614401858304298No Hit
TGTCACTCTCATCGTTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG20.11614401858304298No Hit
CGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG20.11614401858304298No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT20.11614401858304298No Hit
TGTCACTCTCATCGCTGTCGTGGCACCCCGGGAACAGCTTCCCCGCGCGG20.11614401858304298No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGAGAACAGCTTCCCCGCGCGG20.11614401858304298No Hit
TGTCACTCTCCTCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG20.11614401858304298No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCGGCGCGG20.11614401858304298No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACGGCTTCCCCGCGCGG20.11614401858304298No Hit
TGTCACTCTCATCGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.11614401858304298Clontech Universal Primer Mix Long (100% over 23bp)
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT20.11614401858304298No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGCG20.11614401858304298No Hit
TGTCACTCTCATCGCGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC20.11614401858304298No Hit
CAAGTGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTG20.11614401858304298No Hit
TGTCACTATCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG20.11614401858304298No Hit
TGTCACTCTCATCGCTGTCGTGGCACGCCGGGAACAGCTTCCCCGCGCGG20.11614401858304298No Hit
TGTCCCTCTCATCACTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG20.11614401858304298No Hit
TGTCACCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC20.11614401858304298No Hit
TGTCACTCTCATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTC20.11614401858304298No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAAGAGCTTCCCCGCGCGG20.11614401858304298No Hit
TGTCACTCTCATCGCTGACGTGGCACACCGGGAACAGCTTCCCCGCGCGG20.11614401858304298No Hit
TGTCACTCTCAGCGCGGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG20.11614401858304298No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCACCGCGCGG20.11614401858304298No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCTCCGCGCGG20.11614401858304298No Hit
TGCCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG20.11614401858304298No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT20.11614401858304298No Hit
GTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC20.11614401858304298No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT20.11614401858304298No Hit
CAAGTGTAAGCCTCGGCAAGCAAGGCCTGGCTTGTCCCCCCAGGTGTGTG20.11614401858304298No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTACCCGCGCGG20.11614401858304298No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCTGGAACAGCTTCCCCGCGCGG20.11614401858304298No Hit
TGTCACTCCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC20.11614401858304298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)