FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493
Total Bases361.4 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG135254.231849177697555No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA66826.795026073004415No Hit
TGGGTCATGTGGTTCGACAGCGATGAGAGTGACACTGTTCAGACCAAGTC240.9626955475330927No Hit
CTTCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA220.8824709185720016No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGTG170.681909346169274No Hit
CTTCCAGGCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA160.6417970316887284No Hit
TGGGTCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCA110.4412354592860008Clontech SMARTer II A Oligonucleotide (95% over 23bp)
CTTCCAGGCCTCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG90.36101083032490977No Hit
TGGGTCATGTGGTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG80.3208985158443642No Hit
TGGGTCATGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC80.3208985158443642Clontech SMARTer II A Oligonucleotide (95% over 21bp)
CTTCCAGGCCTCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA80.3208985158443642No Hit
TGGGTCATGTGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG80.3208985158443642No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA80.3208985158443642No Hit
CTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA70.2807862013638187No Hit
TGGGTCATGTGGTTCGGAGAGTACATGGAAGCAGTGGTATCAACGCAGAG60.24067388688327318Clontech Universal Primer Mix Long (100% over 22bp)
TGGGTCATGTGGTTCGGAGACGACAAATTCTCAGTGGTGTGTGTTGAGAA50.20056157240272765No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCCCCCAGCAGGG50.20056157240272765No Hit
TGGGTCATGTGGTTCGGCAGCGATGAGAGTGACACTGTTCAGACCAAGTC50.20056157240272765No Hit
TGGGTCAGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG50.20056157240272765No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCCCCTCCTCCGAGCTCCCAGCAGGG50.20056157240272765No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGAGGTGTGTGTTGAGAA40.1604492579221821No Hit
TTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG40.1604492579221821No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCGGGG40.1604492579221821No Hit
TGGGTCATGTGGTTCGGAGACGGCAAGTTCTCAGTGGTGTGTGTTGAGAA40.1604492579221821No Hit
TGGGTCTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG40.1604492579221821No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGGA40.1604492579221821No Hit
CTTCCAGGCCTCCAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA40.1604492579221821No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCAAGCTCCCAGCAGGG40.1604492579221821No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGGGCTCCCAGCAGGG40.1604492579221821No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCGGTGGTGTGTGTTGAGAA40.1604492579221821No Hit
TGGGTCATTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG30.12033694344163659No Hit
CTTCCAGGCCTCCTAGTGCTCTAAGCTCCTCCTCCGAGCTCCCAGCAGGG30.12033694344163659No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCAAGCAGTG30.12033694344163659No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCCCCTCCGAGCTCCCAGCAGGG30.12033694344163659No Hit
TGGGTATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG30.12033694344163659No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGTTCCTCCTCCGAGCTCCCAGCAGGG30.12033694344163659No Hit
CCTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG30.12033694344163659No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGATGAGAA30.12033694344163659No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAACTCCCAGCAGGG30.12033694344163659No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)