FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493
Total Bases360.3 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG113645.56758924989972No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG59823.987164059366226No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT281.1231448054552748No Hit
CAAGTGTAAGCCTCGGCAACTCTGCGTTGATACCACTGCTTCCATGTACT261.0429201764941836Clontech Universal Primer Mix Long (96% over 25bp)
TGTCACTCTCATCGCTGTCGAACCACATGACCCATCCAACTGTAGAACCA220.8824709185720016No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT180.7220216606498195No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT150.601684717208183No Hit
CAAGTGTAAGCCTCGGCAACCAAGGCCTGGCTTGTCCCCCCAGGTGTGTG140.5615724027276374No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT130.5214600882470918No Hit
TGTCCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG120.48134777376654636No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT100.4011231448054553No Hit
CAAGTGTAAGCCTCGGCAACTCTGCGTTGATACCACTGCTTCCCATGTAC90.36101083032490977Clontech Universal Primer Mix Long (96% over 26bp)
CAAGTGTAAGCCTCAGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG80.3208985158443642No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT80.3208985158443642No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT70.2807862013638187No Hit
CAAGTGTAAGCCTCGGCAACTCTGCGTTGATACCACTGCTGGGAGCTCGG70.2807862013638187Clontech Universal Primer Mix Long (95% over 24bp)
TGTCACTCTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA60.24067388688327318Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAAGTGTAACCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT60.24067388688327318No Hit
TGTCACTCTATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC60.24067388688327318No Hit
CAAGTGTAAGCCTGGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG60.24067388688327318No Hit
TGTCACTCTCATCCCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG60.24067388688327318No Hit
CAAGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT60.24067388688327318No Hit
TGTCATCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC60.24067388688327318No Hit
TGTCACTCTCATCGCTGCCGAACCACATGACCCATCCAACTGTAGAACCA50.20056157240272765No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCTCCAGGTGTGTG50.20056157240272765No Hit
CAAGTGAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT50.20056157240272765No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT50.20056157240272765No Hit
CAAGTGTAAGCCTCGGCAAACAAGGTCTGGCTTGTCCCCCCAGGTGTGTG50.20056157240272765No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCCTGTCCCCCCAGGTGTGTG50.20056157240272765No Hit
CAAGTGTAAGCCGCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG50.20056157240272765No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCCGGCTTGTCCCCCCAGGTGTGTG40.1604492579221821No Hit
TAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG40.1604492579221821No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGTG40.1604492579221821No Hit
CAAGTGTAAGCCTCGGTAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG40.1604492579221821No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAACTTCCCCGCGCGG40.1604492579221821No Hit
TGTCACTCTCATCGCTGTCGTGGCACCCCGGGAACAGCTTCCCCGCGCGG40.1604492579221821No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCAGGCTTGTCCCCCCAGGTGTGTG40.1604492579221821No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGCGTG40.1604492579221821No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGAG40.1604492579221821No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTCGTCCCCCCAGGTGTGTG40.1604492579221821No Hit
TGTCACTCTCATCGCAGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG40.1604492579221821No Hit
CAAGGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT30.12033694344163659No Hit
CAAGTGTAAGCCTCGGCAACTCTGCGTTGAGAAGCTGATGCCGCTGAGCT30.12033694344163659No Hit
CAAGTGTAAGCCTCGGCAACTCTGCGTTGATACCACTGAGCTCGTTTTGC30.12033694344163659Clontech SMART CDS Primer II A (95% over 21bp)
TGTGACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCGGCGCGG30.12033694344163659No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCTTGGCTTGTCCCCCCAGGTGTGTG30.12033694344163659No Hit
CAAGTGTAGGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG30.12033694344163659No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGCTGTGTG30.12033694344163659No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG30.12033694344163659No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGTTTGTCCCCCCAGGTGTGTG30.12033694344163659No Hit
TGGCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG30.12033694344163659No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTATGTG30.12033694344163659No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCTCCCCAGGTGTGTG30.12033694344163659No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGATGTGTG30.12033694344163659No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)