FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6594
Total Bases955.9 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG501175.99332726721262No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT761.1525629360024265No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT731.1070670306339097No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT490.743099787685775No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT460.6976038823172581No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT320.48528965726417955No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT250.3791325447376403No Hit
CAAGGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT230.34880194115862906No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGCCCCCCCAGGTGTGTG220.33363663936912347No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGCGTG220.33363663936912347No Hit
TGTCACTCTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA210.3184713375796179Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAAGTGTAAGCCTCGGCAAACAAGGCCCGGCTTGTCCCCCCAGGTGTGTG210.3184713375796179No Hit
TAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG210.3184713375796179No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGTG200.3033060357901122No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGAGTG180.27297543221110104No Hit
CAAGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT180.27297543221110104No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT170.2578101304215954No Hit
CAAGTGTAACCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT160.24264482863208978No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG160.24264482863208978No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGCG150.22747952684258416No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTCGTCCCCCCAGGTGTGTG140.21231422505307856No Hit
CAAGTGAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT120.18198362147406735No Hit
TGTCACTCTCATCGCTGTCGAACCACATGACCCATCCAACTGTAGAACCA120.18198362147406735No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT110.16681831968456173No Hit
CAAGTGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTG110.16681831968456173No Hit
CAAGTGTAAGCCACGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG110.16681831968456173No Hit
CAAGAGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG100.1516530178950561No Hit
CAAGTGTAAGCTTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG100.1516530178950561No Hit
CAAGTGTAAGCCTCGGCAAACAAGGTCTGGCTTGTCCCCCCAGGTGTGTG100.1516530178950561No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCCTGTCCCCCCAGGTGTGTG100.1516530178950561No Hit
CAAGTGTAAGCCTCGGCAAGCAAGGCCTGGCTTGTCCCCCCAGGTGTGTG100.1516530178950561No Hit
CAAGTGTAAGCCGCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG90.13648771610555052No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGACTTGTCCCCCCAGGTGTGTG80.12132241431604489No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCCGGTGTGTG80.12132241431604489No Hit
CAAGTGTAAGCCTCGGCAAACGAGGCCTGGCTTGTCCCCCCAGGTGTGTG80.12132241431604489No Hit
CAAGTGTAAGCCTCGGCAAACAAGACCTGGCTTGTCCCCCCAGGTGTGTG70.10615711252653928No Hit
CAAGTGTTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG70.10615711252653928No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCTCCAGGTGTGTG70.10615711252653928No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTAGCTTGTCCCCCCAGGTGTGTG70.10615711252653928No Hit
CAAGTGTACGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG70.10615711252653928No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGAG70.10615711252653928No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGTTTGTCCCCCCAGGTGTGTG70.10615711252653928No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTATGTG70.10615711252653928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)