FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2053
Total Bases296.4 kbp
Sequences flagged as poor quality0
Sequence length136-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG78638.285435947394056No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG66832.53774963468096No Hit
TGTCACTCTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA452.1919142717973696Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGTCACTCTCATCGCTGTCGAACCACATGACCCATCCAACTGTAGAACCA211.0228933268387725No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT150.7306380905991232No Hit
TGTCACTCTCATCGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT130.6332196785192401Clontech Universal Primer Mix Long (100% over 23bp)
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT110.535801266439357No Hit
TGTCCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG100.4870920603994155No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG60.2922552362396493No Hit
TGTCACTCTCTCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC60.2922552362396493No Hit
TGTACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC50.24354603019970775No Hit
TGTCACTCTCATCGCTGTCGTGGCACCCCGGGAACAGCTTCCCCGCGCGG50.24354603019970775No Hit
TGTCACTCTCATCGCTGTCGTGGCACGCCGGGAACAGCTTCCCCGCGCGG50.24354603019970775No Hit
TGTCACTCTCATCGCTGTCGCGGCACACCGGGAACAGCTTCCCCGCGCGG40.1948368241597662No Hit
CAAGTGTAAGCCTCGGAGGCCTGGAAGTCCAACTGTAGAACCATGTCGTC40.1948368241597662No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT40.1948368241597662No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTCGTCCCCCCAGGTGTGTG40.1948368241597662No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT40.1948368241597662No Hit
TGTCACTCTCAACGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG40.1948368241597662No Hit
TGTCACTCTCATCGCTGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA40.1948368241597662Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG40.1948368241597662No Hit
TGTCATCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC40.1948368241597662No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT40.1948368241597662No Hit
CAAGTGTAAGCCTCGGCAAACAAGACCTGGCTTGTCCCCCCAGGTGTGTG30.14612761811982464No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCTGCGCGG30.14612761811982464No Hit
TGTACTCTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC30.14612761811982464Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT30.14612761811982464No Hit
TAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG30.14612761811982464No Hit
TTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC30.14612761811982464No Hit
TGTCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC30.14612761811982464No Hit
TGTCACTCTCATGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC30.14612761811982464No Hit
CAAGTGTAAGCCTCGGCAAACAAGGTCTGGCTTGTCCCCCCAGGTGTGTG30.14612761811982464No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCGCGCGGC30.14612761811982464No Hit
CAAGTGTAAGCCTGGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG30.14612761811982464No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGGGG30.14612761811982464No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCTG30.14612761811982464No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCTCCCCAGGTGTGTG30.14612761811982464No Hit
TGTCACTCTCATCGTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC30.14612761811982464No Hit
GTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC30.14612761811982464No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGTTTCCCCGCGCGG30.14612761811982464No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGAGTG30.14612761811982464No Hit
TGTCACTCTCATCGCTGTCGTGGCACACTGGGAACAGCTTCCCCGCGCGG30.14612761811982464No Hit
TGTCACTCTCATCCCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG30.14612761811982464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)