FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6516
Total Bases944.7 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG539882.84223449969306No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA1582.424800491098834No Hit
CTTCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA681.043585021485574No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA530.8133824432166974No Hit
CTTCCAGGCCTCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG290.44505831798649476No Hit
CTTCCAGGCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA270.4143646408839779No Hit
CTTCCGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA230.3529772866789441No Hit
CTTCCAGGCCTCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA220.3376304481276857No Hit
CTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA210.32228360957642727No Hit
TGGGTCATGTGGTTCGACAGCGATGAGAGTGACACTGTTCAGACCAAGTC200.3069367710251688No Hit
TTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG180.2762430939226519No Hit
CTTCCAGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA170.2608962553713935No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCCCCGAGCTCCCAGCAGGG160.24554941682013504No Hit
CTTCCAGGCCTCCTAGTGCCCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG150.23020257826887663No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCCCCTCCGAGCTCCCAGCAGGG140.21485573971761818No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCCCCCAGCAGGG140.21485573971761818No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCCCCTCCTCCGAGCTCCCAGCAGGG140.21485573971761818No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCGGGG130.19950890116635975No Hit
CTTCTAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG100.1534683855125844No Hit
CTTCCAGGCCTCCTAGTGCTCTAGACTCCTCCTCCGAGCTCCCAGCAGGG100.1534683855125844No Hit
CTTCCAGGCCTCCTTGACCTTGGGCTTCTCGTCCCCCCATGTACTCTGCG100.1534683855125844No Hit
CATCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG90.13812154696132595No Hit
CTTCCAGGCCTCCTAGTGCTCTAAGCTCCTCCTCCGAGCTCCCAGCAGGG90.13812154696132595No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAACTCCCAGCAGGG90.13812154696132595No Hit
CTTCCAGGCCTCCAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA90.13812154696132595No Hit
CTTCCAGGCCTCCTGGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG80.12277470841006752No Hit
TGGGTCATGTGGTTCGGCAGCGATGAGAGTGACACTGTTCAGACCAAGTC80.12277470841006752No Hit
CTTCCAGGCCTCCTTGACCTTGGGCTTCTCTGCGTTGATACCACTGCTTC70.10742786985880909Clontech SMART CDS Primer II A (95% over 24bp)
CTTCCAGGCCTCCTGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA70.10742786985880909No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCACCCAGCAGGG70.10742786985880909No Hit
CTTCCAGGCCTCCTTGACCTTGGGCTTCTGCGTTGATACCACTGCTTCCC70.10742786985880909Clontech Universal Primer Mix Long (95% over 24bp)
CTTCCAGGCCTCCTTGACCTTGGGCTTCTCGTTGATACCACTGCTTCCCA70.10742786985880909No Hit
CTTCCAGGCCCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA70.10742786985880909No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCAAGCTCCCAGCAGGG70.10742786985880909No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)