FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9853
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG762577.38759768598396No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT1461.481782198315234No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT960.9743225413579619No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT630.6393991677661626No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT580.5886532020704354No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT540.5480564295138536No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT430.4364153049832538No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGCCCCCCCAGGTGTGTG380.38566933928752667No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT330.33492337359179947No Hit
CAAGGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT270.2740282147569268No Hit
CAAGTGAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT270.2740282147569268No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGTG250.25372982847863595No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGCGTGTG250.25372982847863595No Hit
CAAGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT240.24358063533949048No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT240.24358063533949048No Hit
TGTCACTCTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA230.2334314422003451Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGAGTG230.2334314422003451No Hit
CAAGTGTAAGCCTCGGCAAACGAGGCCTGGCTTGTCCCCCCAGGTGTGTG220.22328224906119964No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCGGGTGTGTG200.20298386278290878No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCCTGTCCCCCCAGGTGTGTG190.19283466964376333No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGAG180.1826854765046179No Hit
CAAGTGTAAGCCTGGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG170.17253628336547244No Hit
CAAGTGTAAGCCTCGGTAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG170.17253628336547244No Hit
CAAGTGTAAGCCTCGACAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG170.17253628336547244No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGCG170.17253628336547244No Hit
CAAGTGTAACCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT160.162387090226327No Hit
CAAGTGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTG160.162387090226327No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGCGTG160.162387090226327No Hit
CAAGTGTTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG140.14208870394803613No Hit
CAAGAGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG130.1319395108088907No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGACCCCCCAGGTGTGTG130.1319395108088907No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGACTTGTCCCCCCAGGTGTGTG130.1319395108088907No Hit
CAAGTGTAAGCCTCGGCAAACAAGGTCTGGCTTGTCCCCCCAGGTGTGTG130.1319395108088907No Hit
CAAGTGTAAGCCTCTGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG130.1319395108088907No Hit
CAAGTGTAAGCCTCGGCAAACAAGACCTGGCTTGTCCCCCCAGGTGTGTG120.12179031766974524No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCTAGGTGTGTG120.12179031766974524No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCAGGCTTGTCCCCCCAGGTGTGTG120.12179031766974524No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCCGGCTTGTCCCCCCAGGTGTGTG110.11164112453059982No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCTCCAGGTGTGTG100.10149193139145439No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCTCCCCAGGTGTGTG100.10149193139145439No Hit
CAAGTGTAAGCCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG100.10149193139145439No Hit
CAAGTGTACGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG100.10149193139145439No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTCGTCCCCCCAGGTGTGTG100.10149193139145439No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGTTTGTCCCCCCAGGTGTGTG100.10149193139145439No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)