FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8193
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG566469.13218601244965No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG4885.956304162089589No Hit
TGTCCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG1211.47687049920664No Hit
TGTCACTCTCATCGCTGTCGTGGCACCCCGGGAACAGCTTCCCCGCGCGG460.5614549005248383No Hit
TGTCACTCTATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC430.5248382765775662No Hit
TGTCACTCTCATCGCTGTCGTGACACACCGGGAACAGCTTCCCCGCGCGG430.5248382765775662No Hit
TGTCACTCTCATCCCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG420.5126327352618089No Hit
TGTCACTTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC360.43939948736726475No Hit
TGTCATCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC330.4027828634199927No Hit
TGTACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC290.3539606981569633No Hit
TGTCACTCTCATCGCTGTCGTGGCCCACCGGGAACAGCTTCCCCGCGCGG250.3051385328939339No Hit
TGTCCTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC230.28072745026241913No Hit
TGTCACTCTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA220.2685219089466618Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGTCACTCTCTCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC210.25631636763090443No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACGGCTTCCCCGCGCGG210.25631636763090443No Hit
TGTCACTCTCACGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC180.21969974368363238No Hit
TGTCACTCTCATCGCTGTCGTGGTACACCGGGAACAGCTTCCCCGCGCGG180.21969974368363238No Hit
TGTCACTCTCATCGCTGTCGTGGCACGCCGGGAACAGCTTCCCCGCGCGG180.21969974368363238No Hit
TGTCACTCTCATCGCTGTTGTGGCACACCGGGAACAGCTTCCCCGCGCGG180.21969974368363238No Hit
TGTCACTCTCATCGCTGTCGTGGCGCACCGGGAACAGCTTCCCCGCGCGG160.19528866105211767No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCGCGCGGC160.19528866105211767No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAGCAGCTTCCCCGCGCGG150.18308311973636032No Hit
TGTCACTCTCATCGCTGTCGAACCACATGACCCATCCAACTGTAGAACCA150.18308311973636032No Hit
TTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC140.17087757842060297No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGTGG130.15867203710484562No Hit
TGTCACTCTCAACGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG130.15867203710484562No Hit
TGTCACTCTCATCGCTGTCGCGGCACACCGGGAACAGCTTCCCCGCGCGG120.14646649578908824No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGTTTCCCCGCGCGG120.14646649578908824No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGGACAGCTTCCCCGCGCGG120.14646649578908824No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCCTCCCCGCGCGG120.14646649578908824No Hit
TGTCACCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC110.1342609544733309No Hit
TGTCACTCTCATCGCTGTCGTGGCACACTGGGAACAGCTTCCCCGCGCGG110.1342609544733309No Hit
TGTCACTCTCATCGCTTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC110.1342609544733309No Hit
TGTCACTCCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC110.1342609544733309No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGTGCGG110.1342609544733309No Hit
GTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC110.1342609544733309No Hit
CGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG100.12205541315757354No Hit
TGTGACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCGGCGCGG100.12205541315757354No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT100.12205541315757354No Hit
TGTCACTCTCATCGCTGTCGTGGCACATCGGGAACAGCTTCCCCGCGCGG100.12205541315757354No Hit
TGTCACTCTCATCGCTGTCGTGGCACACAGGGAACAGCTTCCCCGCGCGG100.12205541315757354No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCTCCGCGCGG100.12205541315757354No Hit
TGTCACTCTCATCGCAGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGG100.12205541315757354No Hit
TGTCACTCTCATCGCTGCCGTGGCACACCGGGAACAGCTTCCCCGCGCGG100.12205541315757354No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCAGGAACAGCTTCCCCGCGCGG90.10984987184181619No Hit
TGTCACTCTCATCGCTGTCGTAGCACACCGGGAACAGCTTCCCCGCGCGG90.10984987184181619No Hit
TGTCACTCTCATCGTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC90.10984987184181619No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGA90.10984987184181619No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGAGAACAGCTTCCCCGCGCGG90.10984987184181619No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAACTTCCCCGCGCGG90.10984987184181619No Hit
TGCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCCCGCGCGGC90.10984987184181619No Hit
TGTCACTCTCATCGCTGTCGTGGCACACCGGGAACAGCTTCCTCGCGCGG90.10984987184181619No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)