FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6819
Total Bases988.6 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG582385.39375274967004No Hit
CTTCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA701.0265434814488927No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA550.8065698782812729No Hit
CTTCCAGGCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA380.5572664613579704No Hit
CTTCCAGGCCTCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG370.542601554480129No Hit
CTTCCAGGCCTCCTTGACCTTGGGCTTCTCTGCGTTGATACCACTGCTTC300.43994720633523976Clontech SMART CDS Primer II A (95% over 24bp)
CTTCCAGGCCTCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA290.4252822994573985No Hit
CTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA260.38128757882387443No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCCCCTCCTCCGAGCTCCCAGCAGGG220.32262795131250915No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAACTCCCAGCAGGG200.29329813755682654No Hit
CTTCCGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA190.2786332306789852No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCCCCTCCGAGCTCCCAGCAGGG160.23463851004546118No Hit
CATCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG140.20530869628977857No Hit
CTTCCAGGCCTCCTAGTGCTCTAGACTCCTCCTCCGAGCTCCCAGCAGGG140.20530869628977857No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCCCCCAGCAGGG140.20530869628977857No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGGGCTCCCAGCAGGG130.19064378941193721No Hit
CTTCCAGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA120.1759788825340959No Hit
TTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG120.1759788825340959No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGTTCCCAGCAGGG120.1759788825340959No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAACAGGG110.16131397565625458No Hit
CTTCCAGGCCTCCTTGACCTTGGGCTTCTCTGCGTTGATACCACCCCATG110.16131397565625458No Hit
CTTCCAGGCCTCCAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA100.14664906877841327No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCAAGCTCCCAGCAGGG100.14664906877841327No Hit
CTTCCAGGCCTCCTAGTGCTCTGGGCTCCTCCTCCGAGCTCCCAGCAGGG100.14664906877841327No Hit
CTTCCAGGCCTCCTAGCGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG80.11731925502273059No Hit
CTTCCAGGCCTCCTAGTGCCCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG80.11731925502273059No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGTTCCTCCTCCGAGCTCCCAGCAGGG80.11731925502273059No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCGGGG80.11731925502273059No Hit
TGGGTCATGTGGTTCGGAGACGATCAGATACCGTCGTAGTTCCGACCATA70.10265434814488929No Hit
CTTCCAGGCCTCCTGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA70.10265434814488929No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCCCCGAGCTCCCAGCAGGG70.10265434814488929No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGTAGGG70.10265434814488929No Hit
CTTCCAGGCCCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA70.10265434814488929No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCTTCCTCCGAGCTCCCAGCAGGG70.10265434814488929No Hit
CTTCCAGGCCTCCTAGTGCTCCAGGCTCCTCCTCCGAGCTCCCAGCAGGG70.10265434814488929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)