FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575
Total Bases83.3 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA36563.47826086956522No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG386.608695652173912No Hit
TGGGTCATGTGGTTCGACAGCGATGAGAGTGACACTGTTCAGACCAAGTC152.608695652173913No Hit
TGGGTCATGTGGTTCGGAGACGATCAGATACCGTCGTAGTTCCGACCATA71.2173913043478262No Hit
TGGGTCTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG61.0434782608695654No Hit
TGGGTCATGTGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG50.8695652173913043No Hit
TGGGTCATTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG40.6956521739130435No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGATGAGAA40.6956521739130435No Hit
TGGGTCATGTGGTCCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA40.6956521739130435No Hit
TGGGTCATGTGGTTCGGAGGCTTACACTTGCTGTTCAGACCAAGTCTCTG30.5217391304347827No Hit
TGGGTCATGTGGTTCGGAGACGACAAATTCTCAGTGGTGTGTGTTGAGAA30.5217391304347827No Hit
TGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG30.5217391304347827No Hit
TGGGTCATGGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG30.5217391304347827No Hit
CTTCCAGGCCTCCTTGACCTTGGGCTTCTCTGCGTTGATACCACTGCTTC20.34782608695652173Clontech SMART CDS Primer II A (95% over 24bp)
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAG20.34782608695652173No Hit
TGGGTCATGTGGTTGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG20.34782608695652173No Hit
CTTCCAGGCCTCCTGAATGATATATAAGGCATAAAAGCAAGATGGGACTA20.34782608695652173No Hit
CTTCCAGGCCTCCTTGACCTTGGGCTTCTCTGCGTTGATACCACCCCATG20.34782608695652173No Hit
TGGGTATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG20.34782608695652173No Hit
TGGGTCATGTGGTTCCGAGGCTTACACTTGCTGTTCAGACCAAGTCTCTG20.34782608695652173No Hit
TGGGTCATGTGGTTCGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG20.34782608695652173No Hit
TGAGTCATGTGGTATGATCAGTAAAGATAACTGGGAGAATAGTTAAGAAG10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATACTCAGTGGTGTGTGTTGAGAA10.17391304347826086No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCAAGCAGGG10.17391304347826086No Hit
TGGGTCATGTGGTTCGACAGCGATGAGAGGACACTGTTCAGACCAAGTCT10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGGGGGGCAGAGTCCCAAGGCCAAGAAGAAGGC10.17391304347826086No Hit
TGGGTCATGTGGTTCGGGCTCCTGGACCTTGGGCTTCTCTGCGTTGATAC10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACGGCAATTCTCAGTGGTGTGTGTTGAGAAG10.17391304347826086No Hit
GGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG10.17391304347826086No Hit
TGGTCATGTGGTTCGACAGCGATAGAGTGACACTGTTCAGACCAAGTCTC10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGGAGTTTAACCTTGTAGGAATATTTAAGAGAC10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCACAGTGGTGTGTGTTGAGAA10.17391304347826086No Hit
TGGGTCATGTGGTTCGGGGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACGTCAAATTCTCAGTGGTGTGTGTTGAGAA10.17391304347826086No Hit
CTTCCAGGCCTCCTAGACCTTGGGCTTCTCTGCGTTGATACCCCCATGTA10.17391304347826086No Hit
CTTCCAGGCCTCCTAGTGCTCGACTCCCCTGCAGCCCGGCAAGCTCCCAG10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGATGAGGTGGGGGTAGGCCTGATAGGGCACAG10.17391304347826086No Hit
CTTCCAGCCTCCTACCTTAGCCAGAAAGGGGAGAAGGGTGGGTAGTAACT10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGCGGTGTGTGTTGAGAA10.17391304347826086No Hit
CTTCCAGGTCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACTTCAAATTATCAGTGGTGTGTGTTGAGAA10.17391304347826086No Hit
GGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAGC10.17391304347826086No Hit
CTTCCAGGCCTCCTAGTGCTCTAAGCTCCTCCTCCGAGCTCCCAGCAGGG10.17391304347826086No Hit
TGGGTCATGTTGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA10.17391304347826086No Hit
TGGGTCAGTGGTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAGC10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACGGCAATTTCTCAGTGGTGTGTGTTGAGAA10.17391304347826086No Hit
CTTCCAGGCCTCCTAGTGCTCCTCCCCATGTACTCTGCGTTGATACCACT10.17391304347826086Clontech SMART CDS Primer II A (95% over 22bp)
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGAGGTGTGTGTTGAGAA10.17391304347826086No Hit
TGGGTCATGTGGTTCGGACAGGGAGGCAGGGAGGCAGGGAGAGACAGAGA10.17391304347826086No Hit
CTTCCAGGCCTCCTAGTGCTTCTCAAAAACAAACAAGAACTTTCCAAGCA10.17391304347826086No Hit
CTTCCAGGCCTCCTAGTGCTCCTGGACCTTGGGCTTCTCTGCGTTGATAC10.17391304347826086No Hit
TGGGTCATGTGGTTCAGATAATTTGCCCTGGAAAGGACGGATAGTGACTT10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGAGTTGAGAA10.17391304347826086No Hit
TGGGTCTGTGGGTTTGTTAGGTACTGTTTACATTAATAAATTAAAGCTCC10.17391304347826086No Hit
TGGGTCATGTGTTCGACAGCGATGAGAGTGACACTGTTCAGACCAAGTCT10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAAGAATTTCAGATACATGTTTTCTCCTGTGAAA10.17391304347826086No Hit
TGGGTCATGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTC10.17391304347826086No Hit
TGGGTCGTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA10.17391304347826086No Hit
TGGGTCATGTGGTTGGGATTAACTGGGAAACAGCACAAGGGAACTTGCTG10.17391304347826086No Hit
TGGGTCATGTGGTTCGGGAGAGTGCAGAAGGTTTGAAATAGTTGATTATA10.17391304347826086No Hit
CTTCCAGGCCTCCTAGTGCACTACAAAGTCTGTCACGAAGCTGCATGATA10.17391304347826086No Hit
CTTCCAGGCCTCCTAGTGCGAGGAGGGTGGATGGAATTAAGGGTGTTAGT10.17391304347826086No Hit
TGGGTCATGTGGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGA10.17391304347826086No Hit
CTTCCAGGCCTCCTTGACCTTGGTGGCATTCTCTGCGTTGATACCACTCC10.17391304347826086No Hit
TGGGTCAGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGCGTGTGTTGAGAA10.17391304347826086No Hit
TGGGTCATGTGGTTCTGAGACGGCAAATTCTCAGTGGTGTGTGTTTAGAA10.17391304347826086No Hit
CTTCCAGGCCTCCCAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG10.17391304347826086No Hit
TGGGTCATGTGGTTTGATCAGTAAAGAAAACTGGGAGAATAGTTAAGAAG10.17391304347826086No Hit
TGGGTCATGTGGTTGGAGACGGCAAATTCTCAGTGGTGTGCGTTGAGAAG10.17391304347826086No Hit
TGGGTCATGGGCGGTGGCGACGGCACCGCCCCCGCGTCTCCCTGAGCGGG10.17391304347826086No Hit
TGGGTCATGTGGTGACTTGAGTTTTGGCAATTTGGCTTTCCCCAAGCCGC10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTCTGAGATGAGAA10.17391304347826086No Hit
TGGGTATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGAGTGTTGAGAAG10.17391304347826086No Hit
TGGGTCATGTGGTTCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCA10.17391304347826086No Hit
CTTCCTGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG10.17391304347826086No Hit
TGGGTCATGTGGTCCGACAGCGGTGAGAGTGGCACTGTTCAGACCAAGTC10.17391304347826086No Hit
TGGGTCATGTGGTTGGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA10.17391304347826086No Hit
CTTCCAGGCCTCCTAGTGCTGTGAGAAAATAAGCCTCTGTTGTCCATGGA10.17391304347826086No Hit
CTTCCAGGCCTCCTCGTGCCCCCCCCCCGCATGCACTTTGGCTTAAAGGC10.17391304347826086No Hit
TGGGTCATGTGGTTATATGCTTGTATTTGAGAATGGGAGACTAAAGACCA10.17391304347826086No Hit
CTTCCAGGCCTCCTACCTTAGCCAGAACGGGGAGAAGGGTGGGTAGTAAC10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCCCAGTGGTGTGTGTTGAGAA10.17391304347826086No Hit
TGGGTCATGTGGTATCAACGCAGAGTACATGGGGGGCTATCAACGCAGAG10.17391304347826086Clontech SMARTer II A Oligonucleotide (95% over 21bp)
TGGGTCATGTGGTTCGGAGATGGCAAATTCTCAGTGGTGCGTGTTGAGAA10.17391304347826086No Hit
TGGGCCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGTTGGAGACCAGCCTGGCCAACATGGTGAAAT10.17391304347826086No Hit
TGGGACATGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTC10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGCGTGTTGAGAA10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGGGGCTGGGAGAGGGAGGTAGAAGGATGGGGA10.17391304347826086No Hit
TTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG10.17391304347826086No Hit
TGGGTCATGTGGTTCGGCAGCGATGAGAGTGACACTGTACAGACCAAGTC10.17391304347826086No Hit
TGGGTCATAGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG10.17391304347826086No Hit
CTTCCAGGCCCCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG10.17391304347826086No Hit
TGGGTCATGTGGTTCGCCGAGGCTTACACTTGCTGTTCAGACCAAGTCTC10.17391304347826086No Hit
TGGGTCATGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAG10.17391304347826086Clontech SMARTer II A Oligonucleotide (95% over 21bp)
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCGGTGGTGTGTGTTGAGAA10.17391304347826086No Hit
TGGTCATGTGGTTCGACAGCGATGAGAGTGACACTGTTCAGACCAAGTCT10.17391304347826086No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGATAAAACACATAAAA10.17391304347826086Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGGTATGTGGTTCGACAGCGATGAGAGTGACACTGTTCAGACCAAGTCT10.17391304347826086No Hit
CTTCCAGGCCTCCTAGTGCTCTCCTTGACCTTGGGCTTCTCAGCGTTGAT10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCTGTGGTGTGTGTTGAGAA10.17391304347826086No Hit
TGGGTCATGTGGTTTGGAGACGGCGAATTCTCAGTGGTGTGTGTTGAGAA10.17391304347826086No Hit
TGAGTCATGTGGTATGATCAGTAAAGAAAACTGGGAGAATAGTTAAGAAG10.17391304347826086No Hit
TGGGTCATGTGGTTCGGCTTACACTTGCTGTTCAGACCAAGTCTCTGCTA10.17391304347826086No Hit
TGGGTCATGTGGTTAGGAGATGGCAAATTCTCAGTTGTGTGTGTTGAGAA10.17391304347826086No Hit
TGGGTCATGTGTCCAATAGGTGACACACACTACCTGCTTCACAAGGACCT10.17391304347826086No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCGGGG10.17391304347826086No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA10.17391304347826086No Hit
CTTCCAGGCCTCCTAGTGCTCGTGTTCGGGAAGAGATTGCTGCTGCTGCT10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACTGCAAATTCACAGAGGTGTGTGTTGAGAA10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGTATGAGGCCAGCCTGCCCAACATGGTGAAAC10.17391304347826086No Hit
CTTCCAGGCCTCCTAGGCTTACACTTGCTGTTCAGACCAAGTCTCTGCTA10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGTA10.17391304347826086No Hit
TGGGTCATGTGGTTGACAGCGTGAGAGTGACACTGTTCAGACCAAGTCTC10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGTGTGTGTTGAGAAG10.17391304347826086No Hit
CTTCCAGGCCTCCTTGACCTTGGGCTTCTCTGCGTTGATACCACTGCCCA10.17391304347826086Clontech SMART CDS Primer II A (95% over 22bp)
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGGGAA10.17391304347826086No Hit
TGGGTCATGTGGTTCGGAGTCCACAGAGCTGAGCTTTTGAGCAGACTCTG10.17391304347826086No Hit
CTTCCAGGCCTCCTAGACCTTGGGCTTCTCTGCGTTGATACCACCCCATG10.17391304347826086No Hit

[WARN]Adapter Content

Adapter graph