FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118
Total Bases17 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACTCTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA2622.033898305084744Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGTCACTCTCATCGCTGTCGAACCACATGACCCATCCAACTGTAGAACCA108.47457627118644No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG86.779661016949152No Hit
TGTCACTCTCATCGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT75.932203389830509Clontech Universal Primer Mix Long (100% over 23bp)
TGTCACTCTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGAA32.5423728813559325Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGTCACTATCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA21.694915254237288Clontech Universal Primer Mix Long (96% over 25bp)
TGTCACTCTCATCGCTGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA21.694915254237288Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAAGTGTAAGCCTCGGCCTAGGTCACGTTGGCCCTCAAAGCGAGCCGTTG21.694915254237288No Hit
TGTCACTCTCATCGCAGAGTACATGGAAGCAGTGGTAACAACGCAGAGTA21.694915254237288Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGTCACTCGCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTC21.694915254237288Clontech Universal Primer Mix Long (96% over 25bp)
CAAGTGTAAGCCTCTACAGCTCGTCCTTGACCTTGGGCTTCTCTGCGTTG10.847457627118644No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCCACGCAGAGTA10.847457627118644Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAAGTGGTATCAACGCAGAGAACATGGGTGCCGAAGTAACAAGCCAGAAT10.847457627118644Clontech SMARTer II A Oligonucleotide (95% over 21bp)
TGTCACTCTCATCACATGACCCATCCAACTGTAGAACCATGTCGTCAGTG10.847457627118644No Hit
TGTCACTCTCATCGCTGTCGAACACGTGACCCATCCAACTGTAGAACCAT10.847457627118644No Hit
CAAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT10.847457627118644Clontech Universal Primer Mix Long (96% over 25bp)
CAAGTGGTATCAACGCAGAGTACATGGAAGAAGTGGTATCAACGCAGAGT10.847457627118644Clontech SMARTer II A Oligonucleotide (95% over 23bp)
TGTGACCGCCGCGGGGGGGGGGGGGGGGGGGGGGGCGGGTTGGTTGGGGG10.847457627118644No Hit
TGTCACGCTCATCGCTGTCGAACCACATGACCCATCCAACTGTAGAACCA10.847457627118644No Hit
CGTCACTATCATCGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.847457627118644Clontech Universal Primer Mix Long (96% over 26bp)
TGTCACTCTCATCGCAGAGTACATGGAAGCAGTGGAATCAACGCAGAGTA10.847457627118644Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATACACGCAGAGTA10.847457627118644Clontech SMARTer II A Oligonucleotide (100% over 22bp)
TGTCACTTCATCGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.847457627118644Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCCACGCAGAGT10.847457627118644Clontech SMARTer II A Oligonucleotide (95% over 23bp)
CAAGTGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGTAGCAGTG10.847457627118644Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTCATCTCATCGCTGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC10.847457627118644Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTCACTCTCATCGCAGAGTACATGGGGTGGTATCAACGCAGAGTAGCTG10.847457627118644No Hit
TGTCATCAACGCAGAGTACATGGAAGCAGTGGTTTCCACGCAGAGTACAA10.847457627118644No Hit
TGTCATTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA10.847457627118644Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGTAACAACGCAGAGAACAATGAAGCAGTGGTAACAACTCAGAGAACAT10.847457627118644No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT10.847457627118644No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT10.847457627118644No Hit
TGTCACTCTCATCGCTGTCGAACACATGACCCATCCAACTGTAGAACCAT10.847457627118644No Hit
TGGTATCAACGCAGAGTACATGTAAGCAGTGGGATCAACGCAGAAGAAAT10.847457627118644Clontech SMARTer II A Oligonucleotide (95% over 21bp)
TGTCATCTCATCGCAGAGTACATGGAAGAAGTGGTATCAACGCAGAGTAC10.847457627118644Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGTCACTCTCATCGCAGAGTACATGGTAGCAGTGGTATAAACGCAGAGTA10.847457627118644No Hit
CAGTGTATCAACGCAGAGTACATGGAAGCAGTGGTATCCACGCAGAGAAC10.847457627118644Clontech SMARTer II A Oligonucleotide (95% over 22bp)
TGTCACTCTCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA10.847457627118644Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAAGTGGTATCACCGCAGAGTACATGGAAGCAGTGGGATCAACGCAGAGT10.847457627118644Clontech SMARTer II A Oligonucleotide (95% over 23bp)
TGTCACTCTCATCGCAGAGTACATGCAAGCAGTGGTATCAACGCAGAGTA10.847457627118644Clontech Universal Primer Mix Long (96% over 26bp)
TGTCACTTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC10.847457627118644Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTCACTGTCATCGCAGAGTACAAGGAAGCAGTGGTATCAACGCAGAGTA10.847457627118644Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGTCATCTATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA10.847457627118644Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTCACTCTCATCGCTGCCGAACCACATGACCCATCCAACTGTAGAACCA10.847457627118644No Hit
CAAGTGTAAGCCTCGGCAACAGGAGGGAGACCCTGTCTCAAAAAAAAAAA10.847457627118644No Hit
TGTCCCTCTCATCGCTGTCGAACCACATGACCCATCCAACTGTAGAACCA10.847457627118644No Hit
CAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAACGCAGAGTA10.847457627118644Clontech Universal Primer Mix Long (96% over 25bp)
CAAGTGTAAGCCTCGGCTCACTGCAACCTTGCTTAAACCCAATATTTATA10.847457627118644No Hit
TGTCACTCTCATCGCAGAGTACATGGAAGCAGCGGTATCAACGCAGAGTA10.847457627118644Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGTCACTCTCATCGCAGAGTACATGGAAGCAGTGGTATAAACGCAGAGTA10.847457627118644Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.847457627118644Clontech Universal Primer Mix Long (100% over 22bp)
TGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGAACAT10.847457627118644Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGTCACTCTCATCGCTGTCGTAAGCACTAGGAGGCCTGGAAGTCCAACTG10.847457627118644No Hit
TGTCACTCTCATCGCAGAGTACATGAAAGCAGTGGTATCAACGCAGAGTA10.847457627118644Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TTCACTATCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC10.847457627118644Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAGGTAAGCCGTTAGGTGTAGGCGCAGCGAAAGCGAGTCTGAATAGGGC10.847457627118644No Hit
CAGTGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC10.847457627118644Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAT10.847457627118644Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTCCCTCTCATCGCAGAGTTCATGGAAGCAGTGGTATCAACGCAGAGTA10.847457627118644Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGTGTGAGTCAACCGAGTGTTGTCCAATTGTAACACAAAAAAACTCATAA10.847457627118644No Hit
TGTCACTCTCATCCCTGACGAACCACATGACCCATCCAACTGTAGAACCA10.847457627118644No Hit
TGTCACTCTCATCGCTGCCGAACCACATGACCCATCCAAATGTAGAACCA10.847457627118644No Hit
TGTCACTCTCATCGCAGAGTACATGGAAGCAGTGGTATGAACGCAGAGTA10.847457627118644Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAG10.847457627118644Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph