FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10258
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG510049.717293819457986No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA359435.03606940924156No Hit
CTTCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA550.5361668941314096No Hit
TGGGTCATGTGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG500.48742444921037237No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA410.3996880483525054No Hit
CTTCCAGGCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA380.370442581399883No Hit
CTTCCAGGCCTCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG370.36069409241567557No Hit
TGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG360.35094560343146813No Hit
TGGGTCTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG360.35094560343146813No Hit
CTTCCAGGCCTCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA290.282706180542016No Hit
TGGGTATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG250.24371222460518618No Hit
TGGGTCATGTGGTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG240.23396373562097877No Hit
TGGGTCATGTGGTTGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG220.21446675765256387No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCCCCTCCGAGCTCCCAGCAGGG220.21446675765256387No Hit
CTTCCAGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA190.1852212906999415No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCCCCTCCTCCGAGCTCCCAGCAGGG190.1852212906999415No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGGGAA190.1852212906999415No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGCGTGTGTTGAGAA160.15597582374731916No Hit
TTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG160.15597582374731916No Hit
TGGGTCATTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG160.15597582374731916No Hit
CTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA160.15597582374731916No Hit
TGGGTCAGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG160.15597582374731916No Hit
TGGGCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG150.1462273347631117No Hit
CTTCCGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA150.1462273347631117No Hit
TGGGTCATGGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG140.13647884577890426No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCCCCCAGCAGGG140.13647884577890426No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGCGTGTTGAGAA140.13647884577890426No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAG130.12673035679469682No Hit
TGGGTCATGTGGTTCGGAGACAGCAAATTCTCAGTGGTGTGTGTTGAGAA130.12673035679469682No Hit
CTTCCAGGCCTCCTAGTGCCCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG120.11698186781048939No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGATGAGAA120.11698186781048939No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCCCAGTGGTGTGTGTTGAGAA120.11698186781048939No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCGGGG110.10723337882628194No Hit
GGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG110.10723337882628194No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGAGGTGTGTGTTGAGAA110.10723337882628194No Hit
TGGGTCATGTGGTTCGGAGACGGCAAGTTCTCAGTGGTGTGTGTTGAGAA110.10723337882628194No Hit
TGGGTCATGTGGTTCGGAGACGGCGAATTCTCAGTGGTGTGTGTTGAGAA110.10723337882628194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)