FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690
Total Bases100 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG52976.66666666666667No Hit
CAAGTGTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT81.1594202898550725No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT71.0144927536231882No Hit
CAAGTGTAAGCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT40.5797101449275363No Hit
TGTCACTCTCATCGCTGTCGAACCACATGACCCATCCAACTGTAGAACCA40.5797101449275363No Hit
CAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT40.5797101449275363No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGTGTGTGT30.43478260869565216No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCCTGTCCCCCCAGGTGTGTG30.43478260869565216No Hit
CAAGTGTAGGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG20.2898550724637681No Hit
CAAGTGTAAGCCTGGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG20.2898550724637681No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGACCCCCCAGGTGTGTG20.2898550724637681No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTAGCTTGTCCCCCCAGGTGTGTG20.2898550724637681No Hit
CAAGTGTAAGCCTCCGAACCACATGACCCATCCAACTGTAGAACCATGTC20.2898550724637681No Hit
CAAGGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT20.2898550724637681No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCCGGTGTGTG20.2898550724637681No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCAGGCTTGTCCCCCCAGGTGTGTG20.2898550724637681No Hit
CAAGTGTATGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG20.2898550724637681No Hit
CAAGTGTAAGCCTGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT20.2898550724637681No Hit
CAAGTGAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT20.2898550724637681No Hit
CAAGTGTAAGCCTCGGCTAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG20.2898550724637681No Hit
CAAGTGTAAGCCCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT20.2898550724637681No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGCG20.2898550724637681No Hit
TGTCACTCTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA10.14492753623188406Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAAGTTTAAGCCACGGCAAACAAGGCCTGTCAAGTCCCCCCAGGTGAGTG10.14492753623188406No Hit
TGTCACTCTCATCGCAGACGAACCAAAAGACCCATCCAACTGAAGAAACA10.14492753623188406No Hit
CAAGTGTAAGTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGTT10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGAG10.14492753623188406No Hit
AAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGCCCCCCCAGGTGTGTGT10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCAGGGGTGGGT10.14492753623188406No Hit
TGTCACTCTCATCGCTGTCCAACCACATGACCCATCCAACTGTAGAACCA10.14492753623188406No Hit
CAAGTTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
GTAGGTTCGGGTGGGCCAAGTGCCGGCCACCCCGGCCCCCCCGGGGGGTG10.14492753623188406No Hit
AAAGTGAGAGCCTCGGCAAACAAGGCCTGGCTGGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGGG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCGGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCGCCCAAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTTGGAGGCCTGGAAGTCCAACTGTAGAACCATGTCGTC10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTACCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTTAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTCTAAGGGACGCCAAGCCCTAATGGCACCACGCCCACCACGCAAAT10.14492753623188406No Hit
CAACTGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAATCCTCGGCAAACAAGGCCTGGCTTGTCACCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTCTAAGCCACGGCAAACAAGGCCTGTCAAGTCCCCCCACGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCGGGCTTGTCGCCCCAGGTGGGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCATGTGTGTGT10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAGACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT10.14492753623188406No Hit
CAAGTGTAAGCCTCGGAAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTT10.14492753623188406No Hit
CAAGGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTGT10.14492753623188406No Hit
AAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCAACAAGGTGTGTG10.14492753623188406No Hit
CAAGTGTTTGGGTGGGGTTTGTTGGGGACGGATGTGGGGGCAGGTCTGTC10.14492753623188406No Hit
TGTCACTCTCATCGCTGCCGAACCACATGACCCATCCAACTGTAGAACCA10.14492753623188406No Hit
CAAGTCATTGCCACCCCATTGTAATTAACATAAAACACCACACGCCGTTA10.14492753623188406No Hit
CTACTGTTAGCCTCGGCAAACAAGGCCTGGCTGCTCCCCCCAGGAGTGAG10.14492753623188406No Hit
TGTCCCTCTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA10.14492753623188406Clontech Universal Primer Mix Long (96% over 25bp)
CAAGTGTAAGCCTCGGCAAACACGGCCTGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGACTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCCAGGTGTGT10.14492753623188406No Hit
CAAGTGTAAGGGTGGGGTTTGTTGGGGTGGGTTGTGGGGGGTGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTATG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAACAGAGCAAGACTCGGTCTCAAAAAAAAAAAA10.14492753623188406No Hit
TGTAACTCTCATCGCTGCCGGAACACAAGACCCATCCAACAGTAGAACGA10.14492753623188406No Hit
CAGTGTAAGCCTCGGCAAACAAGGCTTGGCTTGTCCCCCCAGGTGTGTGT10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGCCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCATCGGCAAACAAGGCCTGGCATGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTCTAAGCCACGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
TGTCACTCTCATCGCTGTCGCCCCCTGAAGTGCATTCCCCAGCCAGCCGT10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCGTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT10.14492753623188406No Hit
TGTCACTCTCATCGCTGTCGAACCACATGACCCATCCAACTGTAGAACTA10.14492753623188406No Hit
CAAGTGGAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCGCCAGGTGTGCC10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTACCCCCAGGAGTGTG10.14492753623188406No Hit
CAAATGTAAGCCTCGCCAAACAAGAACTAGCTTGTCCCCCAAGGAGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCNGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCCGGCAAACAAGGGGGGTTTGGGGGGGGGTGGGGTGTGG10.14492753623188406No Hit
CAAGTGAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGTT10.14492753623188406No Hit
CAAGTAAGCCTCTACAGCTCGTCCTTGACCTTGGGCTTCTCTGCGTTGAT10.14492753623188406No Hit
CAAGTGGCCGCACCAACCCTGCCATTCCCCACCCGCGCCCCGTCGCGGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTTCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT10.14492753623188406Clontech Universal Primer Mix Long (96% over 25bp)
CAAGTGTAAGCCTCGCCAAACAAAGCCTGGCTTATCCCCCCAGGTGTGTG10.14492753623188406No Hit
TGCCACTCTCATCGCTGCCGAACCACATACCCATCCAACTGTAGAACCAT10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGCGTG10.14492753623188406No Hit
CAGTGAAAGCCTCGGCCAGGATCAGCTCCACCAGGCTGAAGAGCTCCGGG10.14492753623188406No Hit
CTAGTGTTAGCCTCGCCAAACAAGGCCTGGCTGGTACCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCGCGGCAAACAAGGCCTGGCTTGTCCCCCCAGTTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGAG10.14492753623188406No Hit
CAATGTAAGCCTCGGCAAACAGGGCCTGGCTTGTCCCCCCAGGTGTGTGT10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGACTGGCTTGTCCCCACAGGTGTGTG10.14492753623188406No Hit
CAAGTTAAAGCCACGCCAAACAAAGCATGTCAAATCACCCCAGCTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCACCCAGGAGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGTCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTTTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCTCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTCGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCACGCCAAACAAGGCCAGGCTTCTCCCCCCAGGAGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGTCTGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGGTTTGTTGGGGTGGCTTGTGCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAGCAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCCTAGGTCACGTTGGCCCTCAAAGCGAGCCGTTG10.14492753623188406No Hit
AATGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGTT10.14492753623188406No Hit
CAAGTGAAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGAGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCCGGCAAGCAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT10.14492753623188406No Hit
TGTCACTTCATCGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC10.14492753623188406Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAGTGTTAGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCA10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGAGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGTAAACAAGGCCTGGCTTGTCCCCCCAGTTGTGTG10.14492753623188406No Hit
CATGTGGAAGCCTCGGCAACCCAGGCCTGGCTTGTCCCCCCAGGTGTGGC10.14492753623188406No Hit
CAAGTGTTTGGGTGGGGAAAGTTGGCGTGCCTTGTCGCGGCTGGTGTGTG10.14492753623188406No Hit
CAAGTGTAATCCTCGGCAAACAAGTCCTGTCTTGTCCCCCCAGGTGACTG10.14492753623188406No Hit
CAAGTGTGAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTGT10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCGAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGTGTAAGCCTCGGCAAACATGGCCCGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAAGAGTAAGCCTGGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
CAACTGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGTG10.14492753623188406No Hit
TGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAT10.14492753623188406Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTCACTCTCATCGCTGTCGCCAGGCTGGAGTGCAGTGGCACGACCTCTG10.14492753623188406No Hit
CAACAGTGAGCCTCGCCAAACAAGGCCTGGCTGGTCCCCCCAGGTGAGTG10.14492753623188406No Hit
CAAGGTAAGCCTCGGCAAACAAGGCCTGGCTTGTCCCCCCAGGTGTGGGT10.14492753623188406No Hit
CAAGTGTTTGCTTTCGTTAAGTTCCCGTCGGTTGTCGCCCCTGGTGTGTG10.14492753623188406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)