FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008129858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008129858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10846
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG464042.780748663101605No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA462542.64244882906141No Hit
CTTCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA640.5900792919048498No Hit
TGGGTCATGTGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG580.53475935828877No Hit
TGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG510.4702194357366771No Hit
TGGGTCTGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG370.3411395906324913No Hit
CTTCCAGGCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA360.331919601696478No Hit
TTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA350.32269961276046466No Hit
CTTCCAGGCCTCCTAGTGTTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGG330.3042596348884381No Hit
CTTCCAGGCCTCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA330.3042596348884381No Hit
TGGGTCATTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG300.2765996680803983No Hit
TGGGTATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG290.267379679144385No Hit
TGGGTCATGTGGTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG270.24893970127235848No Hit
TGGGTCAGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG260.2397197123363452No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGCGTGTGTTGAGAA240.22127973446431862No Hit
CTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA230.21205974552830537No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGAGGTGTGTGTTGAGAA200.18439977872026553No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGGGAA190.17517978978425225No Hit
TGGGTCATGGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG170.1567398119122257No Hit
TGGGTCATGTGGTCCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA160.14751982297621244No Hit
TGGGCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG150.13829983404019916No Hit
TGGGTCATGTGGTTGGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA150.13829983404019916No Hit
TGGGTCATGTGGTTGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGAAG140.12907984510418588No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCCCAGTGGTGTGTGTTGAGAA140.12907984510418588No Hit
TGGGTCATGTGGTTCGGAGACGACAAATTCTCAGTGGTGTGTGTTGAGAA130.1198598561681726No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTTGAGGA130.1198598561681726No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATCCTCAGTGGTGTGTGTTGAGAA130.1198598561681726No Hit
CTTCCAGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGCAGGGA120.11063986723215931No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCTCCTCCGAGCTCCCAGTAGGG120.11063986723215931No Hit
TGGGTCATGTGGTTCGGAGACAGCAAATTCTCAGTGGTGTGTGTTGAGAA120.11063986723215931No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGCGTTGAGAA120.11063986723215931No Hit
CTTCCAGGCCTCCTAGTGCTCTAGGCTCCCCCTCCGAGCTCCCAGCAGGG120.11063986723215931No Hit
TGGGTCATGTGGTTCGGAGACGGCAAATTCTCAGTGGTGTGTGTAGAGAA110.10141987829614604No Hit
TGGGTCATGTGGTTCGGGGACGGCAAATTCTCAGTGGTGTGTGTTGAGAA110.10141987829614604No Hit
TGGGTCATGTGGTTCGGAGACGGCGAATTCTCAGTGGTGTGTGTTGAGAA110.10141987829614604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)